PXD027171
PXD027171 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | methylation sites of Histone H2B catalyzed by LaeA |
Description | In Penicillium oxalicum, histone H2B (PDE_01489) was one of the interacting proteins with the methyltransferase LaeA. It suggests that histone H2B may be a direct target of LaeA/Lae1. To verify whether histone H2B is one of the LaeA targets, an in vitro catalytic experiment was performed using Homo sapiens recombinant histone H2B (HsH2B, expressed in Escherichia coli, without histone modification), SAM as substrates, and LaeA. The products were analyzed by LC-MS/MS for histone modifications. Results showed that the mono and di-methylation of lysine 108 and the mono-methylation of lysine 116 were detected. |
HostingRepository | iProX |
AnnounceDate | 2021-07-08 |
AnnouncementXML | Submission_2021-07-07_21:21:59.987.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuqi Qin |
SpeciesList | scientific name: Penicillium oxalicum 114-2; NCBI TaxID: 933388; |
ModificationList | monomethylated residue; dimethylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-07-07 21:20:54 | ID requested | |
⏵ 1 | 2021-07-07 21:22:00 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: histone H2B, histone methylation, LaeA, histone methyltransferase |
Contact List
Lushan Wang | |
---|---|
contact affiliation | Shandong university |
contact email | lswang@sdu.edu.cn |
lab head | |
Yuqi Qin | |
contact affiliation | Shandong university |
contact email | qinyuqi@sdu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |