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PXD027168

PXD027168 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAffinity Purification Mass Spectrometry Analysis of Mutant and Wildtype SARS-CoV-2 Nucleocapsid Protein
DescriptionThe COVID-19 pandemic is arguably the most important global threat to humankind that the world has seen in a century. SARS-CoV-2 has now reached 196 countries across all continents with over 159 million confirmed cases with 3.3 million deaths todate. Currently, our knowledge on how and why SARS-CoV-2 causes severe COVID-19 is continually evolving. Here, we identified three consecutive SNPs (G28881A, G28882A, G28883C) resulting in the Nucleocapsid (N) protein amino acid mutations R203K and G204R (simply termed KR mutation) by large-scale SARS-CoV-2 genome sequencing from clinical swab samples of patients. The nucleocapsid (N) protein of SARS-CoV-2, the highly abundant structure protein within the infected cells, serves multiple functions during viral infection, which besides RNA binding, playing essential roles in viral transcription, replication, and translation. We investigated the impact of this KR mutation on the functional properties of N protein in terms of host protein interaction and phosphorylation status by mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2023-04-11
AnnouncementXMLSubmission_2023-04-11_06:28:03.933.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMuhammadShuaib
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-07 09:24:41ID requested
12021-12-02 11:34:30announced
22023-04-11 06:28:04announced2023-04-11: Updated project metadata.
Publication List
Mourier T, Shuaib M, Hala S, Mfarrej S, Alofi F, Naeem R, Alsomali A, Jorgensen D, Subudhi AK, Ben Rached F, Guan Q, Salunke RP, Ooi A, Esau L, Douvropoulou O, Nugmanova R, Perumal S, Zhang H, Rajan I, Al-Omari A, Salih S, Shamsan A, Al Mutair A, Taha J, Alahmadi A, Khotani N, Alhamss A, Mahmoud A, Alquthami K, Dageeg A, Khogeer A, Hashem AM, Moraga P, Volz E, Almontashiri N, Pain A, SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load. Nat Commun, 13(1):601(2022) [pubmed]
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19
submitter keyword: COVID-19, Nucleocapsid Protein, SARS-CoV-2, Data dependent acqusition (DDA), LC-MS/MS
Contact List
MuhammadShuaib
contact affiliationKing Abdullah University of Science and Technology, Pathogen Genomic Group, Thuwal 23500-6900, Kingdom of Saudi Arabia
contact emailmuhammad.shuaib@kaust.edu.sa
lab head
MuhammadShuaib
contact affiliationKing Abdullah University of Science and Technology
contact emailmuhammad.shuaib@kaust.edu.sa
dataset submitter
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Dataset FTP location
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PRIDE project URI
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