PXD027154

PXD027154 is an original dataset announced via ProteomeXchange.

Title	Listeria infection in hepatocytes
Description	Listeria monocytogenes causes severe foodborne illness in pregnant women and immunocompromised individuals. After the intestinal phase of infection, the liver plays a central role in the clearance of this pathogen through its important functions in immunity. However, recent evidence suggests that subpopulations of L. monocytogenes may escape eradication after prolonged infection of hepatocytes, by entering a persistence phase in vacuoles. Here, we examine whether this long-term infection alters hepatocyte defense pathways, which may be instrumental for bacterial persistence. We first established models of Listeria infection in human hepatocyte cell lines HepG2 and Huh7 and in primary mouse hepatocytes (PMH). In these cells, Listeria efficiently enters the persistence stage after a 3-day infection, while inducing a type I (PMH) or type I/III (HepG2) or no (Huh7) interferon response. RNA-seq analysis identified a common signature of long-term Listeria infection on the hepatocyte transcriptome, characterized by overexpression of a set of genes involved in antiviral immunity and under-expression of many acute phase protein (APP) genes, particularly involved in the complement and coagulation systems. The decrease in APP transcript amounts correlated with lower protein abundance in the secretome of infected cells, as shown by proteomics, and also occurred in the presence of APP inducers (IL-6 or IL-1b). The results also suggest that long-term Listeria infection affects lipid metabolism pathways. Collectively, these results reveal that long-term infection with L. monocytogenes profoundly deregulates the innate immune functions of hepatocytes, which could generate an environment favorable to the establishment of persistent infection.
HostingRepository	PRIDE
AnnounceDate	2022-02-18
AnnouncementXML	Submission_2022-02-18_08:49:51.046.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Celine Henry
SpeciesList	scientific name: Listeria monocytogenes EGD; NCBI TaxID: 1334565;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 04:07:17	ID requested
⏵ 1	2022-02-18 08:49:51	announced

Dataset with its publication pending

submitter keyword: Listerai monocytogenes, hepatocytes, LC-MS/MS

Noirot Philippe
contact affiliation	Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
contact email	philippe.noirot@inrae.fr
lab head
Celine Henry
contact affiliation	INRAE
contact email	celine.henry@inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027154
     1. Label: PRIDE project
     2. Name: Listeria infection in hepatocytes