PXD027153
PXD027153 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Functionality of plant mechanoproteins is based on dual role of cysteines |
| Description | Forisomes are giant polyprotein complexes that undergo an immense and fully reversible conformational change from a spindle into a plug-like state triggered by Ca2+ and pH changes. Resembling their native function in plant vasculature, forisomes can act as valves to efficiently regulate flow in microfluidic capillaries controlled by electro titration. Heterologous expression in yeast allows large scale production of tailor-made artificial forisomes which e.g. can be equipped with enzymes to generate bio-active surfaces. Herein, we analyzed the astonishing phenomenon of a disintegrating artificial forisome formed by truncated protein upon Ca2+ trigger, which opens new intriguing application perspectives of these protein polymers. We were able to assign this phenomenon to the lack of conserved motif M1 in the truncated MtSEO-F1 protein. Ambiguous redox states of four highly conserved cysteines within motif M1 and their participation in intra- and intermolecular disulfide bridges was identified by mass spectrometry analysis. |
| HostingRepository | jPOST |
| AnnounceDate | 2021-11-08 |
| AnnouncementXML | Submission_2023-02-07_00:01:01.022.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Medicago truncatula; NCBI TaxID: 3880; |
| ModificationList | S-carboxamidomethyl-L-cysteine; unknown modification; unknown modification |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-07-07 04:02:12 | ID requested | |
| 1 | 2021-11-08 01:15:04 | announced | |
| 2 | 2022-09-18 03:45:24 | announced | 2022-09-18: Updated FTP location. |
| ⏵ 3 | 2023-02-07 00:01:01 | announced | 2023-02-07: Updated PubMed. |
Publication List
| Rose J, M, ü, ller B, Groscurth S, Giese J, Eirich J, Finkemeier I, Twyman RM, Pr, ü, fer D, Noll GA, The functionality of plant mechanoproteins (forisomes) is dependent on the dual role of conserved cysteine residues. Int J Biol Macromol, 193(Pt B):1332-1339(2021) [pubmed] |
Keyword List
| submitter keyword: Forisomes, Redox, Cysteins, disulfide bonds, alkylation, MtSEO-F1 |
Contact List
| Gundula A. Noll | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001243/ |
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