PXD027146

PXD027146 is an original dataset announced via ProteomeXchange.

Title	A coilin mutant provides a sensitized system for identifying modifiers of alternative splicing and posttranscriptional gene silencing in Arabidopsis thaliana
Description	Coilin is a scaffold protein essential for the structural integrity of Cajal Bodies, which are non-membranous nuclear organelles that are thought to facilitate assembly and maturation of nuclear RNPs, including spliceosomal snRNPs. To investigate further coilin’s functions in plant cells, and to identify proteins that may functionally interact with coilin, we performed a genetic suppressor screen in Arabidopsis thaliana using a coilin (coi) mutant displaying altered splicing of a GFP pre-mRNA. The modified splicing pattern results in a ‘hyper-GFP’ phenotype in young coi seedlings relative to the intermediate level of GFP in wild-type seedlings. Additionally, in newly emerging leaves of older coi seedlings, the GFP gene frequently undergoes abrupt siRNA-associated posttranscriptional gene silencing that persists during growth. In the suppressor screen, we searched for mutations that subdue one or both of these GFP phenotypes and identified several understudied factors in plants: WRAP53, a putative Cajal body protein; SMU2, a predicted splicing-related factor; and ZC3HC1, an uncharacterized zinc finger protein. All three mutations return the hyper-GFP phenotype of the coi mutant to approximately the intermediate wild-type level. The zc3hc1 mutations in particular induce premature and more extensive posttranscriptional gene silencing similar to mutations in SOP1 and DCL4, which are known modifiers of posttranscriptional gene silencing. Candidate coilin-interacting proteins identified by immunoprecipitation-mass spectrometry include many splicing-related factors, nucleolar proteins, and mRNA export factors. Our results demonstrate the usefulness of the coi mutant to identify new modifiers of alternative splicing and posttranscriptional gene silencing, and suggest diverse roles for coilin in plant cells.
HostingRepository	PRIDE
AnnounceDate	2023-07-27
AnnouncementXML	Submission_2023-07-27_02:02:07.072.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-ChihHsu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 02:16:36	ID requested
⏵ 1	2023-07-27 02:02:07	announced

Dataset with its publication pending

submitter keyword: Arabidopsis,coilin, alternative splicing

MarjoriMatzke
contact affiliation	Institute of Plant and Microbial Biology, Academia Sinica
contact email	marjorimatzke@gate.sinica.edu.tw
lab head
Chuan-ChihHsu
contact affiliation	Academia Sinica
contact email	without1102@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027146
     1. Label: PRIDE project
     2. Name: A coilin mutant provides a sensitized system for identifying modifiers of alternative splicing and posttranscriptional gene silencing in Arabidopsis thaliana