PXD027145 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome and phosphoproteome of hippocampus after focal cerebral ischemia 2 h in mice |
Description | We obtained the profiles of neuronal phosphoproteome after cerebral ischemia onset by isolating mice hippocampus. Hippocampus combined from either ten sham or ten focal cerebral ischemia 2 h mice were lysed, digested, labeled with different TMT tags, then pooled and analyzed by LC/LC-MS/MS. Five percent of the pool was used for whole proteome analysis, and the remaining 95% was subjected to phosphoproteome profiling. In total, we quantified 5,174 proteins and 9,062 phosphopeptides. Interesting, 21 proteins were upregulated and 7 proteins were downregulated in hippocampus lysates of cerebral ischemia 2 h relative to sham base on fold change. S100a9, Alpha-2-HS-glycoprotein (Ahsg), Fibrinogen beta chain (Fga) and Complement Component C3(c3) are the top significantly changed, which were highly consistent with previous reports in cerebral ischemia injury. Using wolfpsort software to analysis the Subcellular Location, 57% of detected proteins were location to extracellular, 15% were cytoplasmic protein, another 11% were transport to nucleus, and the others were location to plasma membranes (10%), mitochondria (4%) and endoplasmic reticulum (3%). Moreover,184 phosphorylation sites of 135 proteins were upregulated and 689 phosphorylation sites of 420 proteins were downregulated in hippocampus during cerebral ischemia 2 h compare with sham operation. Employing wolfpsort software analysis the subcellular location, 50% of phosphorylated proteins were location to nucleus, 26% were cytoplasmic protein, another 16% were transport to plasma membranes, and the others were location to mitochondria (4%), extracellular (3%) and cytoskeleton (1%). Motif analysis showed that 85% were belongs to serine-type phosphorylation, about 14 were threonine-type phosphorylation and 1% were tyrosine-type phosphorylation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-14 |
AnnouncementXML | Submission_2022-03-18_09:32:17.872.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Jiang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-07 02:16:34 | ID requested | |
1 | 2022-03-14 02:45:13 | announced | |
⏵ 2 | 2022-03-18 09:32:18 | announced | 2022-03-18: Updated publication reference for PubMed record(s): 35257887. |
Publication List
Jiang W, Zhang P, Yang P, Kang N, Liu J, Aihemaiti Y, Tu H, Phosphoproteome Analysis Identifies a Synaptotagmin-1-Associated Complex Involved in Ischemic Neuron Injury. Mol Cell Proteomics, 21(5):100222(2022) [pubmed] |
Keyword List
submitter keyword: Proteome |
phosphoproteome |
hippocampus |
cerebral ischemia |
Contact List
Hai jun TU |
contact affiliation | College of Biology, Hunan University, China |
contact email | haijuntu@hnu.edu.cn |
lab head | |
Wei Jiang |
contact affiliation | College of Biology, Hunan University |
contact email | jiangweibio@hnu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027145
- Label: PRIDE project
- Name: Proteome and phosphoproteome of hippocampus after focal cerebral ischemia 2 h in mice