PXD027145

PXD027145 is an original dataset announced via ProteomeXchange.

Title	Proteome and phosphoproteome of hippocampus after focal cerebral ischemia 2 h in mice
Description	We obtained the profiles of neuronal phosphoproteome after cerebral ischemia onset by isolating mice hippocampus. Hippocampus combined from either ten sham or ten focal cerebral ischemia 2 h mice were lysed, digested, labeled with different TMT tags, then pooled and analyzed by LC/LC-MS/MS. Five percent of the pool was used for whole proteome analysis, and the remaining 95% was subjected to phosphoproteome profiling. In total, we quantified 5,174 proteins and 9,062 phosphopeptides. Interesting, 21 proteins were upregulated and 7 proteins were downregulated in hippocampus lysates of cerebral ischemia 2 h relative to sham base on fold change. S100a9, Alpha-2-HS-glycoprotein (Ahsg), Fibrinogen beta chain (Fga) and Complement Component C3(c3) are the top significantly changed, which were highly consistent with previous reports in cerebral ischemia injury. Using wolfpsort software to analysis the Subcellular Location, 57% of detected proteins were location to extracellular, 15% were cytoplasmic protein, another 11% were transport to nucleus, and the others were location to plasma membranes (10%), mitochondria (4%) and endoplasmic reticulum (3%). Moreover,184 phosphorylation sites of 135 proteins were upregulated and 689 phosphorylation sites of 420 proteins were downregulated in hippocampus during cerebral ischemia 2 h compare with sham operation. Employing wolfpsort software analysis the subcellular location, 50% of phosphorylated proteins were location to nucleus, 26% were cytoplasmic protein, another 16% were transport to plasma membranes, and the others were location to mitochondria (4%), extracellular (3%) and cytoskeleton (1%). Motif analysis showed that 85% were belongs to serine-type phosphorylation, about 14 were threonine-type phosphorylation and 1% were tyrosine-type phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-18_09:32:17.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Jiang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 02:16:34	ID requested
1	2022-03-14 02:45:13	announced
⏵ 2	2022-03-18 09:32:18	announced	2022-03-18: Updated publication reference for PubMed record(s): 35257887.

Jiang W, Zhang P, Yang P, Kang N, Liu J, Aihemaiti Y, Tu H, Phosphoproteome Analysis Identifies a Synaptotagmin-1-Associated Complex Involved in Ischemic Neuron Injury. Mol Cell Proteomics, 21(5):100222(2022) [pubmed]

submitter keyword: Proteome

phosphoproteome

hippocampus

cerebral ischemia

Hai jun TU
contact affiliation	College of Biology, Hunan University, China
contact email	haijuntu@hnu.edu.cn
lab head
Wei Jiang
contact affiliation	College of Biology, Hunan University
contact email	jiangweibio@hnu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027145
     1. Label: PRIDE project
     2. Name: Proteome and phosphoproteome of hippocampus after focal cerebral ischemia 2 h in mice