PXD027143

PXD027143 is an original dataset announced via ProteomeXchange.

Title	Establishment of a novel in vitro transcription assay
Description	RNA Polymerase II (Pol II) transcriptional recycling is an underappreciated mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of putative RNA Pol II transcriptional recycling factors and quantitative measurement of transcriptional output from recycled transcriptional components. By combing our in vitro transcription assays with other experiments (e.g. in vivo dynamic ChIP-seq assays), we identified PAF1 complex components as drivers for transcription recycling, revealing a new layer in controlling Pol II-dependent transcription. Our findings also point to RNA Pol II transcription recycling as a mechanism that functions aberrantly in disease states such as cancer, and indicate the potential to target factors such as PAF1 that drive RNA Pol II recycling in cancer.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:14:27.235.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027143
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhong Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 02:16:17	ID requested
⏵ 1	2021-11-03 05:14:27	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, Transcription

Qianben Wang
contact affiliation	Department of Pathology and Duke Cancer Institute, Duke University School of Medicine, Durham, NC 27710, USA
contact email	Qianben.wang@duke.edu
lab head
Zhong Chen
contact affiliation	Duke University
contact email	zhong.chen128@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027143

PRIDE project URI

• PRIDE
  1. PXD027143
     1. Label: PRIDE project
     2. Name: Establishment of a novel in vitro transcription assay