PXD027132

PXD027132 is an original dataset announced via ProteomeXchange.

Title	Casein phoshopeptides identification by partial-, double-dephosphorylation and endoGluC digestion
Description	The aim of the present study was the development of a relatively simple method without enrichment step to detect, in a mixture of peptides and phosphopeptides, a maximum of CPPs, including multi-phosphorylated and large-size CPPs, by using high pressure liquid chromatography (HPLC), high-resolution mass spectrometry (MS) and bioinformatics. A commercial casein hydrolysate was used as model hydrolysate for this purpose. This approach consisted of use of double and partial dephosphorylations of CPPs as well as digestion by EndoGluC protease before peptidomics analysis.
HostingRepository	PRIDE
AnnounceDate	2021-09-29
AnnouncementXML	Submission_2021-09-29_05:03:11.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Deracinois
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-06 08:58:26	ID requested
⏵ 1	2021-09-29 05:03:12	announced

Deracinois B, Mat, é, os A, Romelard A, Boulier A, Auger J, Baniel A, Ravallec R, Flahaut C, Partial-, Double-Enzymatic Dephosphorylation and EndoGluC Hydrolysis as an Original Approach to Enhancing Identification of Casein Phosphopeptides (CPPs) by Mass Spectrometry. Foods, 10(9):(2021) [pubmed]

submitter keyword: casein phosphopeptides, mass spectrometry, dephosphorylation, endoGluC digestion

Jean-Louis HILBERT
contact affiliation	UMR Transfrontalière BioEcoAgro N° 1158,Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV – Institut Charles Viollette, F-59000 Lille, France.
contact email	jean-louis.hilbert@univ-lille.fr
lab head
Barbara Deracinois
contact affiliation	UMRT BioEcoAgro
contact email	barbara.deracinois@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027132

PRIDE project URI

• PRIDE
  1. PXD027132
     1. Label: PRIDE project
     2. Name: Casein phoshopeptides identification by partial-, double-dephosphorylation and endoGluC digestion