PXD027130 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome profiling of RNF213 depleted cells reveals nitric oxide regulator DDAH1 antilisterial activity |
Description | RNF213 is a large, poorly characterized interferon-induced protein. Mutations in RNF213 are associated with predisposition for Moyamoya disease (MMD), a rare cerebrovascular disorder. Recently, RNF213 was found to have broad antimicrobial activity in vitro and in vivo, yet the molecular mechanisms behind this function remain unclear. Using mass spectrometry-based proteomics and validation by real-time PCR we here report that knockdown of RNF213 leads to transcriptional upregulation of MVP and downregulation of CYR61, in line with reported pro- and anti-bacterial activities of these proteins. Knockdown of RNF213 also results in downregulation of DDAH1, which we discover to exert anti-microbial activity against Listeria monocytogenes infection. DDAH1 regulates production nitric oxide (NO), a molecule with both vascular and antimicrobial effects. We show that NO production is reduced in macrophages from RNF213 KO mice, suggesting that RNF213 controls Listeria infection through regulation of DDAH1 transcription and production of NO. Our findings propose a potential mechanism for the antilisterial activity of RNF213 and highlight NO as a potential link between RNF213-mediated immune responses and the development of MMD. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_05:01:36.273.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fabien Thery |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-06 08:37:03 | ID requested | |
⏵ 1 | 2022-02-17 05:01:37 | announced | |
Publication List
Martina L, Asselman C, Thery F, Boucher K, Delhaye L, Maia TM, Dermaut B, Eyckerman S, Impens F, Proteome Profiling of RNF213 Depleted Cells Reveals Nitric Oxide Regulator DDAH1 Antilisterial Activity. Front Cell Infect Microbiol, 11():735416(2021) [pubmed] |
Keyword List
submitter keyword: Human, infection, bacteria, Listeria, Listeria monocytogenes, nitric oxide, RNF213, DDAH1 |
Contact List
Francis Impens |
contact affiliation | VIB proteomics core, Ghent; VIB-UGent Center for Medical Biotechnology, Ghent; Department of Biomolecular Medicine, Ghent University. |
contact email | francis.impens@vib-ugent.be |
lab head | |
Fabien Thery |
contact affiliation | VIB-UGent |
contact email | fabien.thery91@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027130 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027130
- Label: PRIDE project
- Name: Proteome profiling of RNF213 depleted cells reveals nitric oxide regulator DDAH1 antilisterial activity