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PXD027130

PXD027130 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profiling of RNF213 depleted cells reveals nitric oxide regulator DDAH1 antilisterial activity
DescriptionRNF213 is a large, poorly characterized interferon-induced protein. Mutations in RNF213 are associated with predisposition for Moyamoya disease (MMD), a rare cerebrovascular disorder. Recently, RNF213 was found to have broad antimicrobial activity in vitro and in vivo, yet the molecular mechanisms behind this function remain unclear. Using mass spectrometry-based proteomics and validation by real-time PCR we here report that knockdown of RNF213 leads to transcriptional upregulation of MVP and downregulation of CYR61, in line with reported pro- and anti-bacterial activities of these proteins. Knockdown of RNF213 also results in downregulation of DDAH1, which we discover to exert anti-microbial activity against Listeria monocytogenes infection. DDAH1 regulates production nitric oxide (NO), a molecule with both vascular and antimicrobial effects. We show that NO production is reduced in macrophages from RNF213 KO mice, suggesting that RNF213 controls Listeria infection through regulation of DDAH1 transcription and production of NO. Our findings propose a potential mechanism for the antilisterial activity of RNF213 and highlight NO as a potential link between RNF213-mediated immune responses and the development of MMD.
HostingRepositoryPRIDE
AnnounceDate2022-02-17
AnnouncementXMLSubmission_2022-02-17_05:01:36.273.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFabien Thery
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-06 08:37:03ID requested
12022-02-17 05:01:37announced
Publication List
Martina L, Asselman C, Thery F, Boucher K, Delhaye L, Maia TM, Dermaut B, Eyckerman S, Impens F, Proteome Profiling of RNF213 Depleted Cells Reveals Nitric Oxide Regulator DDAH1 Antilisterial Activity. Front Cell Infect Microbiol, 11():735416(2021) [pubmed]
Keyword List
submitter keyword: Human, infection, bacteria, Listeria, Listeria monocytogenes, nitric oxide, RNF213, DDAH1
Contact List
Francis Impens
contact affiliationVIB proteomics core, Ghent; VIB-UGent Center for Medical Biotechnology, Ghent; Department of Biomolecular Medicine, Ghent University.
contact emailfrancis.impens@vib-ugent.be
lab head
Fabien Thery
contact affiliationVIB-UGent
contact emailfabien.thery91@gmail.com
dataset submitter
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