PXD027128

PXD027128 is an original dataset announced via ProteomeXchange.

Title	Novel recognition site for polyUb and UBL signals in Rpn1
Description	Rpn1 is the largest proteasomal receptor for polyubiquitin and shuttle proteins contains one known binding site for polyubiquitin and shuttle proteins. We used a combination of NMR spectroscopy, photo-crosslinking, mass spectrometry, and mutagenesis,to confirm the presence of additional ubiquitin recognition site on RPN1. This novel site is situated in the N-terminal section of Rpn1, a region previously surmised to be devoid of functionality.
HostingRepository	PRIDE
AnnounceDate	2021-08-09
AnnouncementXML	Submission_2021-08-09_02:27:08.497.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-06 08:36:48	ID requested
⏵ 1	2021-08-09 02:27:08	announced

Dataset with its publication pending

submitter keyword: RPN1, Ubiquitin, BpA, UV-Crosslinking

David Fushman
contact affiliation	Center Biomol. Struct. & Organization Dept Chemistry & Biochemistry University of Maryland College Park, MD 20742-3360 USA
contact email	fushman@umd.edu
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD027128

PRIDE project URI

• PRIDE
  1. PXD027128
     1. Label: PRIDE project
     2. Name: Novel recognition site for polyUb and UBL signals in Rpn1