PXD027109 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Single cell RNA-seq analysis links respiratory dysfunction to an extracellular matrix-specific damage response |
Description | Energy metabolism and extracellular matrix function are closely connected to orchestrate and maintain tissue organization, but the crosstalk is poorly understood. Here, we used scRNA-seq analysis to uncover the importance of respiration for extracellular matrix homeostasis in mature cartilage. Genetic inhibition of respiration in cartilage results in the expansion of a central area of 1-month-old mouse femur head cartilage showing disorganized chondrocytes and increased deposition of extracellular matrix material. scRNA-seq analysis identified a cluster-specific decrease in mitochondrial DNA-encoded respiratory chain genes and a unique regulation of extracellular matrix-related genes in nonarticular chondrocyte clusters. These changes were associated with alterations in extracellular matrix composition, a shift in the collagen/non-collagen protein content and an increase of collagen crosslinking and ECM stiffness. The results demonstrate, based on findings of the scRNA-seq analysis, that respiration is a key factor contributing to ECM integrity and mechanostability in cartilage and presumably also in many other tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_05:00:19.996.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Janica Wiederstein |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-05 08:55:40 | ID requested | |
⏵ 1 | 2022-02-17 05:00:20 | announced | |
Publication List
Bubb K, Holzer T, Nolte JL, Kr, ü, ger M, Wilson R, Schl, ö, tzer-Schrehardt U, Brinckmann J, Altm, ü, ller J, Aszodi A, Fleischhauer L, Clausen-Schaumann H, Probst K, Brachvogel B, Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage. J Biol Chem, 297(4):101224(2021) [pubmed] |
Keyword List
submitter keyword: ECM, cartilage, respiration |
Contact List
Bent Brachvogel |
contact affiliation | Department of Pediatrics and Adolescent Medicine, Experimental Neonatology, Medical Faculty, University of Cologne, Cologne, Germany and Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany |
contact email | bent.brachvogel@uni-koeln.de |
lab head | |
Janica Wiederstein |
contact affiliation | Institute for Genetics/CECAD (AG Krüger) |
contact email | jwieders@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027109
- Label: PRIDE project
- Name: Single cell RNA-seq analysis links respiratory dysfunction to an extracellular matrix-specific damage response