PXD027101

PXD027101 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics unravels molecular networks of neuroprotection conferred by Brahmi extract in Aβ42 -induced toxicity model of Drosophila melanogaster
Description	Ayurvedic drug formulations Bacopa monnieri and Centella asiatica are known to have neuroprotective effects. These have been traditionally used in the treatment of Alzhemeir’s disease, and other neurological deficits. Using pan neuronal Aβ42 model of Drosophila melanogaster, a mass spectrometry based quantitative proteomic analysis platform was used to generate the data on proteins altered in response to the Aβ42 toxicity and restoration of altered proteins by consumption of aqueous extracts of two Ayurvedic drug formulations Bacopa monnieri and Centella asiatica aqueous extract. Quantitative proteomic analysis resulted in 0.67 million mass spectra corresponding to 2,59,168 peptide-spectrum matches (PSM) mapping to 24,305 non- redundant peptides corresponding to 11,480 Drosophila melanogaster proteins. Proteins were filtered for >3 PSMs, resulting in 9,540 proteins. Flies expressing Aβ42 significantly altered 517 proteins which were involved in maintaining essential neuronal functions. Supplementing flies with Bacopa monnieri or Centella asiatica extract commonly rescued 224 proteins from Aβ42 toxicity, moreover, extract supplemented group significantly altered proteins which were additionally supporting neuronal maintenance in flies with Aβ42 stress.
HostingRepository	PRIDE
AnnounceDate	2021-11-10
AnnouncementXML	Submission_2021-11-09_21:34:20.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-05 08:24:11	ID requested
⏵ 1	2021-11-09 21:34:21	announced

Dataset with its publication pending

submitter keyword: Alzheimer's disease, neurodegeneration, quantitative proteomics, neuroprotection, Bacopa monnieri, Centella asiatica, aqueous extract, amyloid toxicity, mitochondrial dysfunction

T. S. Keshava Prasad
contact affiliation	Centre for Systems Biology and Molecular Mechanism, Yenepoya Research Center, Yenepoya (Deemed to be Univeristy), Mangalore, Karnataka, India
contact email	keshav@yenepoya.edu.in
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027101
     1. Label: PRIDE project
     2. Name: Quantitative proteomics unravels molecular networks of neuroprotection conferred by Brahmi extract in Aβ42 -induced toxicity model of Drosophila melanogaster