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PXD027080

PXD027080 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein kinase signalling at the Leishmania kinetochore captured by XL-BioID
DescriptionElucidating protein kinase driven signaling pathways is an important but challenging problem in cell biology. Phosphoproteomics has been used to identify many phosphorylation sites, however the spatial context of these sites within the cell is mostly unknown making it difficult to reconstruct signalling pathways. To address this problem an in vivo proximity capturing workflow was developed consisting of proximity biotinylation followed by protein cross-linking (XL-BioID). This was applied to protein kinases of the Leishmania kinetochore leading to the discovery of a novel essential kinetochore protein, KKT26. XL-BioID enabled the quantification of proximal phosphosites at the kinetochore through the cell cycle, allowing the phosphorylation state of the kinetochore to be followed during assembly. A specific inhibitor of kinetochore protein kinases KKT10/KKT19 was used to show that XL-BioID provides a spatially focussed view of protein kinase inhibition, identifying 16 inhibitor responsive proximal phosphosites, including 3 on KKT2, demonstrating the potential of this approach for discovery of in vivo kinase signalling pathways.
HostingRepositoryMassIVE
AnnounceDate2021-07-08
AnnouncementXMLSubmission_2021-07-08_04:17:41.091.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAdam Dowle
SpeciesList scientific name: Leishmania mexicana; NCBI TaxID: 5665;
ModificationListCarbamidomethyl; Oxidation; Phospho; Acetyl; Biotin
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-04 00:31:41ID requested
12021-07-08 04:17:41announced
Publication List
no publication
Keyword List
submitter keyword: Proximity biotinylation, Proximity labeling, Cross-linking, Kinase, Kinetochore, Leishmania, Phosphorylation, Phosphosite, miniTurboID, BirA, AB1
Contact List
Jeremy C. Mottram
contact affiliationUniversity of York
contact emailjeremy.mottram@york.ac.uk
lab head
Adam Dowle
contact affiliationUniversity of York
contact emailadam.dowle@york.ac.uk
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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