PXD027076

PXD027076 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the pathogen surface in cholera identifies host proteins involved in defense and microbiota recognition
Description	The microbial cell surface is a critical site of microbe-host interactions that often control infection outcomes. Defining the set of host proteins present at this interface has been challenging. Here, we used a surface biotinylation approach coupled to quantitative mass spectrometry to identify and quantify both bacterial and host proteins present on the surface of diarrheal fluid-derived V. cholerae in an infant rabbit model of cholera. Our data showed that V. cholerae surfaces were coated with numerous host proteins, whose abundance were driven by the presence of cholera toxin, including the C type lectin SP-D. Mice lacking SP-D had enhanced V. cholerae intestinal colonization. Additional host proteins (AnxA1, LPO and ZAG) capable of binding V. cholerae were also found to recognize distinct taxa of the murine intestinal microbiota, suggesting that these host factors may play roles in intestinal homeostasis in addition to host defense. Proteomic analysis of microbial surfaces is valuable for identifying host interactions that regulate infection and homeostasis with both pathogens and endogenous microbiota alike.
HostingRepository	PRIDE
AnnounceDate	2021-10-02
AnnouncementXML	Submission_2021-10-02_05:32:07.472.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027076
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Abdelrahim Zoued
SpeciesList	scientific name: Vibrio cholerae O1 biovar El Tor str. N16961; NCBI TaxID: 243277; scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-02 08:51:20	ID requested
⏵ 1	2021-10-02 05:32:07	announced

Dataset with its publication pending

submitter keyword: Vibrio cholerae, oryctolagus cuniculus

Matthew K Waldor
contact affiliation	Harvard Medical School, Brigham and Women's Hospital
contact email	mwaldor@research.bwh.harvard.edu
lab head
Abdelrahim Zoued
contact affiliation	Harvard Medical School - Brigham and Women's Hospital
contact email	a.zoued@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027076
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the pathogen surface in cholera identifies host proteins involved in defense and microbiota recognition