PXD027063

PXD027063 is an original dataset announced via ProteomeXchange.

Title	Multiomics analysis of keratin degradation by Fervidobacterium islandicum AW-1
Description	Firmicutes and dermatophytes degrade recalcitrant insoluble keratins resistant to proteolysis. Such highly ordered peptide fibrils can be digested through proteolysis as well as sulfitolysis. However, its biological mechanism relevant to keratin degradation remains unclear. Here we present genome-wide multi-omics analyses to describe how the extremophilic bacterium Fervidobacterium islandicum AW-1degrades keratin under stress conditions. Physiological and biochemical evidence integrated with multi-omics data reveals a nutrient deprivation-induced metabolic shift, thereby increasing chemotactic persister formation for cellular adhesion to keratins and activating the membrane-associated keratinolysis. Quantitative dynamic gene expression and metabolite profiling confirmed that stringent response-induced flagella synthesis is a prerequisite for biofilm formation and that keratin degradation is responsible for swarming dispersal. This study illustrates the molecular details of keratinolysis, providing insight into the survivability of primordial bacteria coping with scarce nutrition relevant to recurrent infections with superficial pathogens.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_08:55:48.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong-Woo Lee
SpeciesList	scientific name: Fervidobacterium islandicum; NCBI TaxID: NEWT:2423;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-02 08:23:47	ID requested
⏵ 1	2026-03-16 08:55:49	announced

Dataset with its publication pending

submitter keyword: starvation, stringent response, extremophiles, keratinolysis, LC-MS/MS, Fervidobacterium islandicum AW-1

Dong-Woo Lee
contact affiliation	Department of Biotechnology, Yonsei University, Seoul 03722, South Korea
contact email	leehicam@yonsei.ac.kr
lab head
Dong-Woo Lee
contact affiliation	Department of Biotechnology, Yonsei University, Seoul 03722, South Korea
contact email	leehicam@yonsei.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027063
     1. Label: PRIDE project
     2. Name: Multiomics analysis of keratin degradation by Fervidobacterium islandicum AW-1