PXD027054

PXD027054 is an original dataset announced via ProteomeXchange.

Title	The proteome of B cell maturation identifies PDCD4 as limiting the response of marginal zone B cells
Description	During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. We used high-resolution mass spectrometry to quantify the proteome of B cell subsets from the mouse spleen and map the differential expression of environmental sensing, transcription- and translation initiation-factors that define cellular identity and function. By comparing transcriptome and proteome, we identified mRNAs linked to B cell activation and differentiation that are expressed without detectable protein. These "poised" mRNAs might enable rapid protein production through increased translation or protein stability. In addition, we found that the translational repressor PDCD4 restrains the response of marginal zone B cells to a T-independent antigen. Our molecular characterization of B cell maturation is a valuable resource to further explore the mechanisms underpinning the specialised functions of B cell subsets.
HostingRepository	PRIDE
AnnounceDate	2023-06-21
AnnouncementXML	Submission_2023-06-21_05:10:04.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AndrewHowden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-01 23:30:59	ID requested
⏵ 1	2023-06-21 05:10:04	announced

Dataset with its publication pending

submitter keyword: B cells, B cell maturation

MartinTurner
contact affiliation	Laboratory of Lymphocyte Signalling and Development, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT
contact email	martin.turner@babraham.ac.uk
lab head
AndrewHowden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027054
     1. Label: PRIDE project
     2. Name: The proteome of B cell maturation identifies PDCD4 as limiting the response of marginal zone B cells