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PXD027051

PXD027051 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of NK3.3-derived extracellular vesicles
DescriptionOur study investigates the features and anti-tumor function of extracellular vesicles (EVs) isolated from our well described NK cell line - NK3.3 - the only published clonal, normal human cell line to date. Natural killer (NK) cells, critical for proper immune function, destroy tumor cells primarily through the release of granules containing potent cytolytic molecules. NK cells also release these molecules within membrane-bound exosomes and microvesicles collectively known as EVs. We show that NK3.3 EVs contain the cytolytic molecules perforin, granzymes A and B, and granulysin, and an array of common EV proteins. We previously showed that the E3 ubiquitin ligase identified by our laboratory, natural killer lytic-associated molecule (NKLAM/RNF19b), is localized to NK granules and is essential for maximal NK killing; our current study shows NKLAM situated within the NK3.3 EV membrane. Analysis of NK3.3 EV contents also identified multiple RNA species including miRNAs associated with anti-tumor activity. We demonstrate that treatment of an array of hematopoietic and non-hematopoietic tumor cell lines with NK3.3 EVs inhibits proliferation and induces apoptosis and cell death while leaving normal cells unaffected. Taken together, our study suggests that NK3.3 EVs have the potential to be effective immunotherapeutic agents.
HostingRepositoryPRIDE
AnnounceDate2021-09-07
AnnouncementXMLSubmission_2021-09-07_04:13:30.417.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027051
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAllyson Cochran
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF; nanoACQUITY UPLC with HDX Technology
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-01 23:28:59ID requested
12021-09-07 04:13:31announced
Publication List
Cochran AM, Kornbluth J, Extracellular Vesicles From the Human Natural Killer Cell Line NK3.3 Have Broad and Potent Anti-Tumor Activity. Front Cell Dev Biol, 9():698639(2021) [pubmed]
Keyword List
submitter keyword: natural killer cell, extracellular vesicle, NKLAM, RNF19b, NK3.3, ubiquitin ligase, leukemia, exosome, extracellular vesicle
Contact List
Dr. Jacki Kornbluth
contact affiliationDepartment of Pathology, Saint Louis University, St. Louis, Missouri, United States
contact emailjacki.kornbluth@health.slu.edu
lab head
Allyson Cochran
contact affiliationSaint Louis University - Department of Pathology
contact emailallyson.cochran@slu.edu
dataset submitter
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Dataset FTP location
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