PXD027050

PXD027050 is an original dataset announced via ProteomeXchange.

Title	Inhibition of BET proteins during adolescence affects prefrontal cortex development: relevance to schizophrenia
Description	Background: The present study investigated a role of proteins from BET family (epigenetic readers) in schizophrenia-like abnormalities in MAM-E17 model of schizophrenia. Methods: An inhibitor of BET proteins, JQ1, was given during adolescence in postnatal days (P) 23-P30, and behavioral response (sensorimotor gating, recognition memory) and prefrontal cortex (mPFC) function (long-term potentiation (LTP), molecular and proteomic studies) were performed in adult males and females. Results: Deficits in sensorimotor gating and recognition memory were observed only in MAM-treated males. However, adolescent JQ1 treatment affected control, but MAM-treated groups in both sexes. Electrophysiological study showed an LTP impairment only in male MAM-treated animals, and JQ1 did not have any effect on LTP in the mPFC. In contrast, MAM did not affect immediate early gene expression (markers of neuronal and synaptic activity), but JQ1 altered them in both sexes. Proteomic study revealed alterations in MAM-treated groups only in males, while JQ1 affected both sexes. Conclusions: Prenatal MAM administration induced schizophrenia-like abnormalities only in males. In contrast, adolescent JQ1 treatment affected both sexes and induced behavioral changes in control groups, altered a markers of neuronal and synaptic activity and proteomic landscape in the mPFC of both groups (VEH- and MAM-treated). Thus, adolescent inhibition of BET family might change neuroplasticity in the mPFC.
HostingRepository	PRIDE
AnnounceDate	2021-09-07
AnnouncementXML	Submission_2021-09-07_04:13:54.790.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027050
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Przemyslaw Mielczarek
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-01 23:26:39	ID requested
⏵ 1	2021-09-07 04:13:55	announced

Bilecki W, Wawrzczak-Bargie, ł, a A, Majcher-Ma, ś, lanka I, Chmelova M, Ma, ć, kowiak M, Inhibition of BET Proteins during Adolescence Affects Prefrontal Cortical Development: Relevance to Schizophrenia. Int J Mol Sci, 22(16):(2021) [pubmed]

submitter keyword: BET proteins, prefrontal cortex, schizophrenia, mass spectrometry, proteomics, shotgun, MALDI-TOF/TOF

Marzena Mackowiak
contact affiliation	Maj Institute of Pharmacology, Polish Academy of Sciences, Department of Pharmacology, Laboratory of Pharmacology and Brain Biostructure, Smetna Str. 12, 31-343 Krakow, Poland
contact email	mackow@if-pan.krakow.pl
lab head
Przemyslaw Mielczarek
contact affiliation	Department of Analytical Chemistry and Biochemistry, AGH University of Science and Technology, Krakow Poland Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland
contact email	przemyslaw.mielczarek@agh.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027050
     1. Label: PRIDE project
     2. Name: Inhibition of BET proteins during adolescence affects prefrontal cortex development: relevance to schizophrenia