PXD027041

PXD027041 is an original dataset announced via ProteomeXchange.

Title	Proteomics of MSC-derived exosomes upon Plekhm1-KO
Description	Leukemia cells instruct their surrounding bone marrow microenvironment (BMM) rendering it hospitable to leukemia cell survival. Conversely, how cells of the BMM influence leukemia progression is less well understood. Pleckstrin homology domain family M member 1 (PLEKHM1) serves as a hub between fusion and secretion of intracellular vesicles. Here, we performed label-free quantitative proteomics to investigate the exosomal cargo released by BMM-derived mesenchymal stromal cells (MSC) lacking Plekhm1 compared to wild-type cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:22:31.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Tascher
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-01 07:56:32	ID requested
1	2023-01-16 04:08:06	announced
⏵ 2	2023-11-14 07:22:32	announced	2023-11-14: Updated project metadata.

Karantanou C, Minciacchi VR, Kumar R, Zanetti C, Bravo J, Pereira RS, Tascher G, Tertel T, Covarrubias-Pinto A, Bankov K, Pfeffermann LM, Bonig H, Divieti-Pajevic P, McEwan DG, Giebel B, M, ü, nch C, Dikic I, Krause DS, Impact of mesenchymal stromal cell-derived vesicular cargo on B-cell acute lymphoblastic leukemia progression. Blood Adv, 7(7):1190-1203(2023) [pubmed]

submitter keyword: exosome,mesenchymal stromal cells, LFQ, Qexactive HF

Daniela Krause
contact affiliation	Goethe-Universität Frankfurt, Georg-Speyer-Haus, Paul-Ehrlich-Str. 42-44, Frankfurt am Main, GERMANY
contact email	Krause@gsh.uni-frankfurt.de
lab head
Georg Tascher
contact affiliation	Institute of Biochemistry II, Goethe University Hospital Frankfurt/Main, Germany
contact email	tascher@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD027041

PRIDE project URI

• PRIDE
  1. PXD027041
     1. Label: PRIDE project
     2. Name: Proteomics of MSC-derived exosomes upon Plekhm1-KO