PXD027033

PXD027033 is an original dataset announced via ProteomeXchange.

Title	Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.)
Description	Drought is one of the major constraints for crop productivity across the globe. Chickpea (Cicer arietinum L.) is mainly cultivated in the arid and semi-arid tropical regions under rain-fed conditions and drought stress is one of the major constraints, which causes up to 50% yield losses annually. In this study, transcriptomics, proteomics and metabolomics datasets from root tissues of contrasting drought responsive chickpea genotypes, ICC 4958 (drought-tolerant), JG 11 (drought-tolerant); an introgression line, JG 11+ (drought-tolerant) and ICC 1882, (drought-sensitive) under control and stress conditions were generated. The integrated analysis of these multi-omics data revealed complex molecular mechanism underlying drought stress response in chickpea. Transcriptomics integrated with proteomics data identified enhancement of hub proteins encoding isoflavone 4’-O-methyltransferase (Ca_06356), UDP-D-glucose/UDP-D-Galactose 4-epimerase (Ca_15037) and delta-1-pyrroline-5-carboxylate synthesis (Ca_24241). These proteins highlighted the involvement of critical pathways such as antibiotic biosynthesis, galactose metabolism and isoflavonoid biosynthesis in activating drought stress response mechanism. Subsequently, integration of metabolomics data identified six key metabolites (fructose, galactose, glucose, myo-inositol, galactinol and raffinose) that showed enhanced correlation with galactose metabolism. Further, integration of root -omics data together with genomic dataset of the “QTL-hotspot” region harbouring several drought tolerance related traits revealed involvement of candidate genes encoding aldo keto reductase family oxidoreductase (Ca_04551) and leucine rich repeat extensin 2 (Ca_04564). These results from integrated multi-omics approach provided a comprehensive understanding and new insights into the drought stress response mechanism of chickpea.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:01:30.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Palak Chaturvedi
SpeciesList	scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-01 02:35:34	ID requested
1	2023-08-23 07:54:41	announced
2	2023-11-14 07:09:26	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:01:31	announced	2024-10-22: Updated project metadata.

10.1002/TPG2.20337;

submitter keyword: drought stress, signalling pathways, proteomics, metabolomics, integrated multi-omics, gene expression, transcriptomics,chickpea

Palak Chaturvedi
contact affiliation	University of Vienna
contact email	palak.chaturvedi@univie.ac.at
lab head
Palak Chaturvedi
contact affiliation	University of Vienna
contact email	palak.chaturvedi@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027033
     1. Label: PRIDE project
     2. Name: Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.)