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PXD027031

PXD027031 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleWild type enrichment of a 25-phospho-steroid kinase involved in anaerobic steroid degradation
DescriptionThe anaerobic degradation of cholesterol fundamentally differs from the cytochrome P450 monooxygenase dependent activation of the isoprenoid side chain at C26 in aerobes. In the denitrifying β-proteobacterium Sterolibacterium denitrificans, activation of primary C26 proceeds via a three-step cascade involving (i) a water-dependent hydroxylation at tertiary C25, (ii) an ATP-dependent dehydration to an alkene, and (iii) a water-dependent hydroxylation at primary C26 to an allylic alcohol. Here, we enriched a 25-hydroxy-steroid kinase (25-HSK) from Sterolibacterium denitrificans that catalyses the ATP-dependent transformation of 25-OH-cholest-1,4-diene-3-one (25-OH-CDO) to 25-phospho-CDO.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:32:45.525.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027031
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristian Jacoby
SpeciesList scientific name: Sterolibacterium denitrificans; NCBI TaxID: 157592;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-01 02:35:19ID requested
12022-02-17 04:55:18announced
22024-10-22 05:32:51announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD027031;
10.1016/j.jbc.2021.101105;
Jacoby C, Goerke M, Bezold D, Jessen H, Boll M, A fully reversible 25-hydroxy steroid kinase involved in oxygen-independent cholesterol side-chain oxidation. J Biol Chem, 297(4):101105(2021) [pubmed]
Keyword List
submitter keyword: cholesterol,Steroid degradation, kinase
Contact List
Matthias Boll
contact affiliationInstitute of Biology II, Microbiology, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
contact emailmatthias.boll@biologie.uni-freiburg.de
lab head
Christian Jacoby
contact affiliationUniversity of Freiburg
contact emailchristian.jacoby@biologie.uni-freiburg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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Repository Record List
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