PXD027031 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Wild type enrichment of a 25-phospho-steroid kinase involved in anaerobic steroid degradation |
Description | The anaerobic degradation of cholesterol fundamentally differs from the cytochrome P450 monooxygenase dependent activation of the isoprenoid side chain at C26 in aerobes. In the denitrifying β-proteobacterium Sterolibacterium denitrificans, activation of primary C26 proceeds via a three-step cascade involving (i) a water-dependent hydroxylation at tertiary C25, (ii) an ATP-dependent dehydration to an alkene, and (iii) a water-dependent hydroxylation at primary C26 to an allylic alcohol. Here, we enriched a 25-hydroxy-steroid kinase (25-HSK) from Sterolibacterium denitrificans that catalyses the ATP-dependent transformation of 25-OH-cholest-1,4-diene-3-one (25-OH-CDO) to 25-phospho-CDO. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:32:45.525.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD027031 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Christian Jacoby |
SpeciesList | scientific name: Sterolibacterium denitrificans; NCBI TaxID: 157592; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-01 02:35:19 | ID requested | |
1 | 2022-02-17 04:55:18 | announced | |
⏵ 2 | 2024-10-22 05:32:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: cholesterol,Steroid degradation, kinase |
Contact List
Matthias Boll |
contact affiliation | Institute of Biology II, Microbiology, Albert-Ludwigs-University Freiburg, Freiburg, Germany. |
contact email | matthias.boll@biologie.uni-freiburg.de |
lab head | |
Christian Jacoby |
contact affiliation | University of Freiburg |
contact email | christian.jacoby@biologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027031
- Label: PRIDE project
- Name: Wild type enrichment of a 25-phospho-steroid kinase involved in anaerobic steroid degradation