PXD027031

PXD027031 is an original dataset announced via ProteomeXchange.

Title	Wild type enrichment of a 25-phospho-steroid kinase involved in anaerobic steroid degradation
Description	The anaerobic degradation of cholesterol fundamentally differs from the cytochrome P450 monooxygenase dependent activation of the isoprenoid side chain at C26 in aerobes. In the denitrifying β-proteobacterium Sterolibacterium denitrificans, activation of primary C26 proceeds via a three-step cascade involving (i) a water-dependent hydroxylation at tertiary C25, (ii) an ATP-dependent dehydration to an alkene, and (iii) a water-dependent hydroxylation at primary C26 to an allylic alcohol. Here, we enriched a 25-hydroxy-steroid kinase (25-HSK) from Sterolibacterium denitrificans that catalyses the ATP-dependent transformation of 25-OH-cholest-1,4-diene-3-one (25-OH-CDO) to 25-phospho-CDO.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:45.525.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027031
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Jacoby
SpeciesList	scientific name: Sterolibacterium denitrificans; NCBI TaxID: 157592;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-01 02:35:19	ID requested
1	2022-02-17 04:55:18	announced
⏵ 2	2024-10-22 05:32:51	announced	2024-10-22: Updated project metadata.

10.6019/PXD027031;

10.1016/j.jbc.2021.101105;

submitter keyword: cholesterol,Steroid degradation, kinase

Matthias Boll
contact affiliation	Institute of Biology II, Microbiology, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
contact email	matthias.boll@biologie.uni-freiburg.de
lab head
Christian Jacoby
contact affiliation	University of Freiburg
contact email	christian.jacoby@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027031

PRIDE project URI

• PRIDE
  1. PXD027031
     1. Label: PRIDE project
     2. Name: Wild type enrichment of a 25-phospho-steroid kinase involved in anaerobic steroid degradation