PXD027025

PXD027025 is an original dataset announced via ProteomeXchange.

Title	CD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis
Description	We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic analysis showed that the common exosomal markers such as CD63, CD81, CD82, Alix, and TSG101 were all identified in EVs. Statistical analysis of EV protein content showed that the top 10 serum proteins in EVs was significantly decreased by using the CD9-HPLC-IAC method compared to the ultracentrifugation (UC) (p=0.001) and size exclusion chromatography (SEC) (p=0.009), and apolipoproteins significantly reduced 4.8-fold compared to the SEC method (p<0.001). The result demonstrated the potential of the CD9-HPLC-IAC method for efficient isolation and proteomic characterization of EVs from a micro-scale volume of serum.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:24:35.160.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianhui Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 22:35:55	ID requested
1	2022-10-14 03:10:36	announced
⏵ 2	2023-11-14 07:24:40	announced	2023-11-14: Updated project metadata.

Zhu J, Zhang J, Ji X, Tan Z, Lubman DM, Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles. J Proteome Res, 20(10):4901-4911(2021) [pubmed]

submitter keyword: Serum, Immunoaffinity Isolation, CD9,Extracellular vesicles, HPLC, Proteomics

David M. Lubman
contact affiliation	Department of Surgery, University of Michigan, USA
contact email	dmlubman@umich.edu
lab head
Jianhui Zhu
contact affiliation	University of Michigan
contact email	jianhuiz@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD027025

PRIDE project URI

• PRIDE
  1. PXD027025
     1. Label: PRIDE project
     2. Name: CD9-HPLC-IAC Isolation of Serum Extracellular Vesicles for Proteomic Analysis