PXD027020

PXD027020 is an original dataset announced via ProteomeXchange.

Title	Global identification ofphospho-dependent SCF ubiquitin ligase substrate reveal a role of ERK-FBXO22-BAG3 axis in tumorigenesis
Description	293T cells were cultured in SILAC medium. Heavy group was labeled with K6&R10. Light group was labeled with K0&R0. In light group, cells were treated with MLN4924. In heavy group, cells were treated with DMSO. Then cells were lysed, mixed and tryptic digested. Peptide mixture was subjected to phosphopeptide enrichment followed by LC-MS/MS analysis. MS raw data was processed using Maxquant(1.5.3.8) against a human proteome database from Uniprot. BAG3 is one of identified substrate. In order to find molecular machanism of BAG. TMT labeling based quantitative proteomics technique was used. Details were shown in manuscript.
HostingRepository	iProX
AnnounceDate	2021-06-29
AnnouncementXML	Submission_2023-03-02_19:26:28.066.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoji Cong
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 17:49:26	ID requested
⏵ 1	2023-03-02 19:26:29	announced

Liu P, Cong X, Liao S, Jia X, Wang X, Dai W, Zhai L, Zhao L, Ji J, Ni D, Liu Z, Chen Y, Pan L, Liu W, Zhang J, Huang M, Liu B, Tan M, Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis. Cell Death Differ, 29(1):1-13(2022) [pubmed]

submitter keyword: MLN4924,Phosphoproteomics

Minjia Tan
contact affiliation	Shanghai Insititute of Meteria Medica
contact email	mjtan@simm.ac.cn
lab head
Xiaoji Cong
contact affiliation	shanghai institute of materia medica
contact email	congxiaoji@qq.com
dataset submitter

iProX dataset URI