PXD027015

PXD027015 is an original dataset announced via ProteomeXchange.

Title	Determination of carry-over contaminations in recombinant major peanut allergen Ara h 2 produced in Nicotiana benthamiana leaves and analysis of proline hydroxylation patterns
Description	The major peanut allergen Ara h 2 undergoes site-specific hydroxylation at three proline residues within repetitive IgE-binding DPYSPOHS sequences. There has been no report of recombinant Ara h 2 production with hydroxyprolines until now. We produced recombinant Ara h 2 in N. benthamiana leaves to determine host-related contaminants and to analyze site-specific hydroxylation of proline residues.
HostingRepository	PRIDE
AnnounceDate	2023-03-20
AnnouncementXML	Submission_2023-03-20_04:49:27.501.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DorotheaAnrather
SpeciesList	scientific name: Arachis hypogaea var. vulgaris; NCBI TaxID: 925390;
ModificationList	monohydroxylated proline
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 05:13:26	ID requested
1	2021-09-28 00:56:07	announced
⏵ 2	2023-03-20 04:49:28	announced	2023-03-20: Updated project metadata.

Ü, z, ü, lmez Ö, Kalic T, Mayr V, Lengger N, Tscheppe A, Radauer C, Hafner C, Hemmer W, Breiteneder H, Product in Allergy Diagnosis. Front Plant Sci, 12():723363(2021) [pubmed]

submitter keyword: Peanut allergens, hydroxyproline

HeimoBreiteneder
contact affiliation	Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria
contact email	heimo.breiteneder@muv.ac.at
lab head
DorotheaAnrather
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, University of Vienna
contact email	dorothea.anrather@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027015

PRIDE project URI

• PRIDE
  1. PXD027015
     1. Label: PRIDE project
     2. Name: Determination of carry-over contaminations in recombinant major peanut allergen Ara h 2 produced in Nicotiana benthamiana leaves and analysis of proline hydroxylation patterns