PXD027014

PXD027014 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of proteins specifically binding to ac4C RNA probes
Description	As a newly identified mRNA modification, the regulation of ac4C remains largely unexplored. RNA-binding proteins (RBPs) that specifically binds to ac4C modification and mediate downstream cellular activities (readers) have not been reported yet. We synthesized acetylated and non-acetylated RNA probes by in vitro transcription. The sequences of the probes were segments of FUS and 18s rRNA, which contain ac4C sites as reported. A biotin-RNA pulldown assay and mass spectrometry were performed with HEK 293T cell lysates.
HostingRepository	PRIDE
AnnounceDate	2021-07-20
AnnouncementXML	Submission_2021-07-19_21:44:12.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuting Xiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 05:13:10	ID requested
⏵ 1	2021-07-19 21:44:12	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: ac4C, RNA pulldown, acetylated RNA probes

submitter keyword: ac4C, RNA pulldown, acetylated RNA probes

Contact List

Xiaoyan Liang
contact affiliation	Reproductive Medicine Center, the Sixth Affiliated Hospital, Sun Yat-sen University
contact email	liangxy2@mail.sysu.edu.cn
lab head
Yuting Xiang
contact affiliation	the Sixth Affiliated Hospital, Sun Yat-sen University
contact email	xiang_yt@126.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD027014
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD027014

PRIDE project URI

Repository Record List

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