PXD027010

PXD027010 is an original dataset announced via ProteomeXchange.

Title	RUFY3 links Arl8b and JIP4-Dynein complex to regulate lysosome size and positioning
Description	The whole-cell scale spatial organization of lysosomes is regulated by their bidirectional motility on microtubule tracks. Small GTP-binding (G) protein, Arl8b, stimulates the anterograde transport of lysosomes by recruiting adaptor protein SKIP (also known as PLEKHM2), which in turn couples the microtubule motor kinesin-1. Here, we have identified an Arl8b effector, RUN and FYVE domaincontaining protein family member 3, RUFY3, which drives the retrograde transport of lysosomes. Artificial targeting of RUFY3 to the surface of mitochondria was sufficient to drive their perinuclear positioning. We find that RUFY3 interacts with the JIP4-Dynein-Dynactin complex and mediates Arl8b association with the retrograde motor complex. The mobile fraction of the total lysosomes per cell was significantly enhanced upon RUFY3 depletion, suggesting that RUFY3 maintains the lysosomes clustering within the perinuclear cloud. Expectedly, RUFY3 knockdown disrupted the perinuclear positioning of lysosomes upon nutrient starvation and/or serum depletion, although lysosome continued to undergo fusion with autophagosomes. Interestingly, lysosome fission events were more frequent in RUFY3-depleted cells and accordingly, there was a striking reduction in lysosome size, an effect that was also observed
HostingRepository	PRIDE
AnnounceDate	2022-03-23
AnnouncementXML	Submission_2022-03-23_07:15:44.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gaurav kumar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 05:12:54	ID requested
⏵ 1	2022-03-23 07:15:47	announced

Kumar G, Chawla P, Dhiman N, Chadha S, Sharma S, Sethi K, Sharma M, Tuli A, RUFY3 links Arl8b and JIP4-Dynein complex to regulate lysosome size and positioning. Nat Commun, 13(1):1540(2022) [pubmed]

submitter keyword: Lysosome, Small GTPase, Arl8b, Dynein, Microtubule

Amit Tuli
contact affiliation	Department of Cell Biology and Immunnology
contact email	atuli@imtech.res.in
lab head
gaurav kumar
contact affiliation	Senior Research Fellow
contact email	gk92@imtech.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD027010

PRIDE project URI

• PRIDE
  1. PXD027010
     1. Label: PRIDE project
     2. Name: RUFY3 links Arl8b and JIP4-Dynein complex to regulate lysosome size and positioning