PXD027007

PXD027007 is an original dataset announced via ProteomeXchange.

Title	Crotonylation of GAPDH regulates human embryonic stem cell endodermal lineage differentiation and metabolic switch
Description	Post-translational modifications of proteins are crucial to the regulation of their activity and function. As a newly discovered acylation modification, crotonylation of non-histone proteins remains largely unexplored, particularly in human embryonic stem cells (hESCs). Here we report the investigation of induced crotonylation in hESCs, which resulted in hESCs of different pluripotency states differentiating into the endodermal lineage. We showed that increased protein crotonylation in hESCs was accompanied by transcriptomic shifts and decreased glycolysis. Through large-scale profiling of non-histone protein crotonylation, we identified metabolic enzymes as major targets of inducible crotonylation in hESCs. We further discovered GAPDH as a key glycolytic enzyme regulated by crotonylation during endodermal differentiation from hESCs, where crotonylation of GAPDH decreased its enzymatic activity thereby leading to reduced glycolysis. Our study demonstrates that crotonylation of glycolytic enzymes may be crucial to metabolic switching and cell fate determination in hESCs.
HostingRepository	PRIDE
AnnounceDate	2022-10-27
AnnouncementXML	Submission_2022-10-26_23:17:37.462.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JingranZhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	crotonylated L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 05:12:37	ID requested
⏵ 1	2022-10-26 23:17:37	announced

Dataset with its publication pending

submitter keyword: hESCs

ZhouSongyang
contact affiliation	Sun Yat-sen University
contact email	songyangz@mail.sysu.edu.cn
lab head
JingranZhang
contact affiliation	Sun Yat-sen University
contact email	326192479@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027007
     1. Label: PRIDE project
     2. Name: Crotonylation of GAPDH regulates human embryonic stem cell endodermal lineage differentiation and metabolic switch