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PXD026996

PXD026996 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMotif-centric phosphoproteomics to target kinase-mediated signaling pathways
DescriptionIdentifying cellular phosphorylation pathways based on kinase-substrate relationships is a critical step to understanding the regulation of physiological functions in cells. Mass spectrometry-based phosphoproteomics workflows have made it possible to comprehensively collect information on individual phosphorylation sites in a variety of samples. However, there is still no generic approach to uncover phosphorylation networks based on kinase-substrate relationships in rare cell populations. Here, we describe a motif-centric phosphoproteomics approach combined with multiplexed isobaric labeling, in which in vitro kinase reaction is used to generate the targeted phosphopeptides, which are spiked into one of the isobaric channels to increase detectability. Proof-of-concept experiments demonstrate selective and comprehensive quantification of targeted phosphopeptides by using multiple kinases for motif-centric channels. Over 7,000 tyrosine phosphorylation sites were quantified from several tens of µg of starting materials. This approach enables the quantification of multiple phosphorylation pathways under physiological or pathological regulation in a motif-centric manner.
HostingRepositoryjPOST
AnnounceDate2021-12-01
AnnouncementXMLSubmission_2022-11-21_08:24:39.595.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChia-Feng Tsai
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; TMT; TMT; alpha-amino acetylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-29 10:49:31ID requested
12021-12-01 15:02:03announced
22022-09-18 02:49:25announced2022-09-18: Updated FTP location.
32022-11-21 08:24:40announced2022-11-22: Updated PubMed.
Publication List
Tsai CF, Ogata K, Sugiyama N, Ishihama Y, Motif-centric phosphoproteomics to target kinase-mediated signaling pathways. Cell Rep Methods, 2(1):100138(2022) [pubmed]
Keyword List
submitter keyword: Motif centric, Phosphoproteome, Kinase, IMAC
Contact List
Yasushi Ishihama
lab head
Chia-Feng Tsai
contact affiliationPacific Northwest National Laboratory
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001027/