PXD026989

PXD026989 is an original dataset announced via ProteomeXchange.

Title	O-glycoproteome of Tannerella forsythia
Description	Tannerella forsythia is a Gram-negative oral pathogen known to possess an O-glycosylation system responsible for targeting multiple proteins associated with virulence at the three-residue motif (D)(S/T)(A/I/L/V/M/T). Multiple proteins have been identified to be decorated with a decasaccharide glycan composed of a poorly defined core plus a species-specific portion whose biosynthesis is largely characterized. To date, the glycosylation of mainly the two S-layer glycoproteins, TfsA and TfsB has been studied yet the true extent of glycosylation within this species has not been explored. In the present study we explore the glycoproteome of T. forsythia employing FAIMS based glycopeptide enrichment of a cell membrane fraction. We demonstrate that at least 13 glycans are utilized within the T. forsythia glycoproteome varying with respect to the presence of the three terminal sugars and the presence of fucose and digitoxose branches at the reducing end. To improve the localization of glycosylation events and enhance the detection of glycopeptides we applied trifluoromethanesulfonic acid treatment to allow the selective chemical cleavage of glycans. By reducing the chemical complexity of glycopeptides this dramatically improved the number of glycopeptides identified and our ability to localize glycosylation sites by ETD fragmentation leading to the identification of 312 putative glycosylation sites in 145 glycoproteins. Glycosylation site analysis revealed that glycosylation occurs on a much broader glycosylation motif than initially reported with glycosylation found at (D)(S/T)(A/I/L/V/M/T/S/C/G/F). The data confirm earlier predictions of hundreds of possible O-glycoproteins present in this organism.
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_00:22:44.037.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026989
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Veith
SpeciesList	scientific name: Tannerella forsythia; NCBI TaxID: 28112;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-29 03:04:32	ID requested
⏵ 1	2021-10-12 00:22:44	announced

Veith PD, Scott NE, Reynolds EC, Characterization of the O-Glycoproteome of Tannerella forsythia. mSphere, 6(5):e0064921(2021) [pubmed]

submitter keyword: Tannerella forsythia, O-glycosylation, Trifluoromethanesulfonic acid

Eric Reynolds
contact affiliation	Melbourne Dental School, University of Melbourne
contact email	e.reynolds@unimelb.edu.au
lab head
Paul Veith
contact affiliation	Melbourne Dental School
contact email	pdv@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026989
     1. Label: PRIDE project
     2. Name: O-glycoproteome of Tannerella forsythia