PXD026976

PXD026976 is an original dataset announced via ProteomeXchange.

Title	Targeted Proteomics Using Stable Isotope Labeled Protein Fragments Enables Precise and Robust Determination of Total Apolipoprotein(a) in Human Plasma
Description	Lipoprotein(a), also known as Lp(a), is an LDL-like particle composed of apolipoprotein(a) (apo(a)) bound covalently to apolipoprotein B100. Plasma concentrations of Lp(a) are highly heritable and vary widely between individuals. Elevated plasma concentration of Lp(a) is considered as an independent, causal risk factor of cardiovascular disease (CVD). Targeted mass spectrometry (LC-SRM/MS) combined with stable isotope-labeled recombinant proteins provides robust and precise quantification of proteins in the blood, making LC-SRM/MS assays appealing for monitoring plasma proteins for clinical implications. This study presents a novel quantitative approach, based on prototypic peptides, to determine the absolute concentration of apo(a) from two microliters of plasma and qualified according to guideline requirements for targeted proteomics assays. After optimization, assay parameters such as linearity, lower limits of quantification (LLOQ), intra-assay variability (CV: 4.72%) and inter-assay repeatability (CV: 7.76%) were determined and the LC-SRM/MS results were benchmarked against a commercially available immunoassay. In summary, the measurements of an apo(a) single copy specific peptide and a K4 specific peptide allows for the determination of molar concentration and relative size of apo(a) in individuals.
HostingRepository	PanoramaPublic
AnnounceDate	2023-02-17
AnnouncementXML	Submission_2023-02-17_09:37:14.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Andreas Hober
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Altis; 6490 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-28 20:03:27	ID requested
1	2023-02-05 13:52:08	announced
⏵ 2	2023-02-17 09:37:15	announced	: Added PubMed Id

Hober A, Rekanovic M, Forsstr, ö, m B, Hansson S, Kotol D, Percy AJ, Uhl, é, n M, Oscarsson J, Edfors F, Miliotis T, Targeted proteomics using stable isotope labeled protein fragments enables precise and robust determination of total apolipoprotein(a) in human plasma. PLoS One, 18(2):e0281772(2023) [pubmed]

submitter keyword: apolipoprotein(a) (apo(a)), apolipoproteins, quantification, stable isotope labeled recombinant protein fragment standard, liquid chromatography, tandem mass spectrometry

Fredrik Edfors
contact affiliation	Division of Systems Biology, Department of Protein Science, School of Chemistry, Biotechnology and Health, The Royal Institute of Technology (KTH), Stockholm. Sweden
contact email	fredrik.edfors@scilifelab.se
lab head
Andreas Hober
contact affiliation	Division of Systems Biology, Department of Protein Science, School of Chemistry, Biotechnology and Health, The Royal Institute of Technology (KTH), Stockholm. Sweden
contact email	andreas.hober@scilifelab.se
dataset submitter

Panorama Public dataset URI