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PXD026975

PXD026975 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis unraveled paraquat-induced cholesterol biosynthesis dysregulation in human brain microvascular endothelial cells
DescriptionDespite its banishment in several countries, Paraquat (PQ) is still one of the most commonly used herbicides in agriculture. This compound is known to induce damaging effects on human or animal brain cells such as glial cells or neurons through biological processes as redox by generation of Reactive Oxygen Species (ROS). This specific perturbation has already proven its association for increasing the risk of developing a long-term brain impairment in in vitro and in vivo studies using brain cells. However, there is few evidence of PQ effect on Human Brain Microvascular Endothelial Cells (HBMECs), a particular cell type acting as a macromolecule permeability regulator in the Blood-Brain Barrier (BBB). The present study aimed at unraveling biological mechanisms associated to the exposition of 1, 10 and 100 µM of PQ for 24h on HBMECs. High-throughput mass spectrometry-based proteomics using data-independent acquisition (DIA) was applied. Biological pathway enrichment and cellular assays such as mitochondrial respiration and cholesterol level of PQ-treated HBMECs were performed to verify proteomics results. Proteomic experiment reported a total of 3753 quantified proteins out of which 419 were significantly modulated by paraquat exposure. Biological pathway enrichment revealed that the modified proteins are involved in pathways such as the ubiquinone metabolism, a pathway directly linked to mitochondrial complex I proteins. This study also unveiled the modulation of the cholesterol metabolism. In conclusion, most of the highly differentially expressed proteins (DEPs) in PQ-treated HBMECs were associated to the ubiquinone metabolism pathway confirming the well know mechanism of PQ inducing oxidative stress and inhibit mitochondrial complex I activity. Additionally, this study also described the cholesterol biosynthesis modulation on primary human brain endothelial cells not yet described.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:32:49.935.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTatjana Vujic
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetic acid derivatized residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-28 10:08:54ID requested
12022-02-17 05:12:34announced
22024-10-22 05:32:58announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-021-97175-w;
Tatjana V, Domitille S, Jean-Charles S, Paraquat-induced cholesterol biosynthesis proteins dysregulation in human brain microvascular endothelial cells. Sci Rep, 11(1):18137(2021) [pubmed]
Keyword List
submitter keyword: proteomics, DIA, brain endothelial cells, cholesterol,Paraquat, mitochondria
Contact List
Jean-Charles Sanchez
contact affiliationTranslational Biomarker Group, University of Geneva, Geneva, Switzerland
contact emailJean-Charles.Sanchez@unige.ch
lab head
Tatjana Vujic
contact affiliationDepartment of Medicine, University of Geneva, Geneva, Switzerland
contact emailtatjana.vujic@unige.ch
dataset submitter
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Dataset FTP location
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