PXD026974

PXD026974 is an original dataset announced via ProteomeXchange.

Title	Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis
Description	Label-free quantitative proteomics was employed to compare the protein content of extracellular vesicles isolated by various differential centrifugation-based approaches from expressed prostatic secretions in urine (EPS-urine) from men with prostate cancer. The developed optimized approach improved EV purity by depleting the high-abundance urine protein Tamm-Horsfall protein (THP) and other common contaminants and achieved relative enrichment of prostate cancer-associated EV-resident proteins.
HostingRepository	PRIDE
AnnounceDate	2022-02-09
AnnouncementXML	Submission_2022-02-09_02:10:52.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Correll
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-28 10:06:59	ID requested
1	2022-02-09 01:55:49	announced
⏵ 2	2022-02-09 02:10:53	announced	2022-02-09: Updated publication reference for PubMed record(s): 35119778.

Correll VL, Otto JJ, Risi CM, Main BP, Boutros PC, Kislinger T, Galkin VE, Nyalwidhe JO, Semmes OJ, Yang L, Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis. J Extracell Vesicles, 11(2):e12184(2022) [pubmed]

submitter keyword: Human, Extracellular Vesicles, EV, Urine, Expressed Prostatic Secretions in Urine, EPS-Urine, Prostate Cancer, Label-Free, LC-MS/MS

O. John Semmes
contact affiliation	Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School, Norfolk, VA, USA. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA.
contact email	semmesoj@evms.edu
lab head
Vanessa Correll
contact affiliation	Eastern Virginia Medical School
contact email	correlvl@evms.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026974

PRIDE project URI

• PRIDE
  1. PXD026974
     1. Label: PRIDE project
     2. Name: Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis