PXD026965

PXD026965 is an original dataset announced via ProteomeXchange.

Title	Narrow precursor window for DIA-MS enhances protein identification and quantification
Description	Data independent acquistion (DIA) is becoming widely as a method of choice in quantitation proteomics due to its high reproducibility and quantitation accuracy at high-throughput fashion. In this project, we systematically evaluated the effect of the selected precursor mass range for DIA-MS on the protein identification and quantification. We show that a narrow precursor window (400-650 m/z) DIA could identify a 34.7% more proteins than a conventional DIA with a wide precursor window of 400-1200 m/z. When combining the three narrow precursor windows (400-650, 650-900, and 900-1200 m/z) DIA-MS analyses, we obtained a 59.4% increase in the number of proteins quantified than a conventional DIA-MS analysis.
HostingRepository	PRIDE
AnnounceDate	2021-10-01
AnnouncementXML	Submission_2021-09-30_20:44:57.260.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huoming Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-28 04:59:31	ID requested
⏵ 1	2021-09-30 20:44:58	announced

Zhang H, Bensaddek D, Narrow Precursor Mass Range for DIA-MS Enhances Protein Identification and Quantification in Arabidopsis. Life (Basel), 11(9):(2021) [pubmed]

submitter keyword: Arabidopsis, Data independent acqusition (DIA), LC-MS/MS

Huoming Zhang
contact affiliation	King Abdullah University of Science and Technology, Corelabs, Thuwal 23500-6900, Kingdom of Saudi Arabia
contact email	huoming.zhang@kaust.edu.sa
lab head
Huoming Zhang
contact affiliation	King Abdullah University of Science and Technology
contact email	huoming.zhang@kaust.edu.sa
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026965
     1. Label: PRIDE project
     2. Name: Narrow precursor window for DIA-MS enhances protein identification and quantification