PXD026959

PXD026959 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic analysis of cecal contents from healthy mice versus mice with DSS-induced colitis
Description	The gut microbiota influences host epigenetics by fermenting dietary fiber into butyrate. Although butyrate could promote histone acetylation by inhibiting histone deacetylases, it may also undergo oxidation to acetyl-CoA, a necessary cofactor for histone acetyltransferases. Here, we find that epithelial cells from germ-free mice harbor a loss of histone H4 acetylation across the genome except at promoter regions. Using stable isotope tracing in vivo with 13C-labeled fiber, we demonstrate that the microbiota supplies carbon for histone acetylation. Subsequent metabolomic profiling revealed hundreds of labeled molecules and supported a microbial contribution to host fatty acid metabolism, which declined in response to colitis and correlated with reduced expression of genes involved in fatty acid oxidation. These results illuminate the flow of carbon from the diet to the host via the microbiota, disruptions to which may affect energy homeostasis in the distal gut and contribute to the development of colitis.
HostingRepository	PRIDE
AnnounceDate	2022-12-11
AnnouncementXML	Submission_2022-12-11_11:51:34.101.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026959
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	PederLund
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-27 16:15:20	ID requested
⏵ 1	2022-12-11 11:51:34	announced

10.6019/PXD026959;

submitter keyword: DSS, inulin, microbiota, cecal contents, colitis, metaproteomics

Benjamin A.Garcia
contact affiliation	Dept. of Biochemistry and Molecular Biophysics, Epigenetics Institute, University of Pennsylvania. Moving shortly to Dept. of Biochemistry and Molecular Biophysics at Washington University in St. Louis.
contact email	bgarci@pennmedicine.upenn.edu
lab head
PederLund
contact affiliation	University of Pennsylvania
contact email	pederjlund@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026959
     1. Label: PRIDE project
     2. Name: Metaproteomic analysis of cecal contents from healthy mice versus mice with DSS-induced colitis