PXD026953

PXD026953 is an original dataset announced via ProteomeXchange.

Title	Two novel zinc and cobalt-responsive proteins in marine eukaryotic phytoplankton and implications for Zn scarcity in the surface oceans
Description	Observations of scarce surface ocean concentrations of dissolved zinc (Zn) have raised the possibility that Zn may co-limit the growth of oceanic phytoplankton, yet testing this hypothesis has been difficult because Zn is highly prone to contamination. Biomarkers of micro-nutrient stress can serve as an alternate means to assay, though biomarker proteins for Zn stress in marine algae are currently uncharacterized. In this study we identified two Zn-responsive proteins in four marine diatom species that became abundant under Zn and Co limitation, but were scarce or undetectable in replete conditions. We refer to these proteins as ZCRP-A and ZCRP-B (Zn/Co Responsive Protein A and B). Sequence similarity of ZCRP-A to the Escherichia coli model COG0523 protein YjiA suggests a Zn2+ binding capability using conserved metal binding sites. Cellular localization by fluorescent tagging and overexpression of these proteins showed that ZCRP-B is part of a membrane-bound transport complex and that ZCRP-A serves as a Zn/Co chaperone with a chloroplastic target. These results imply that ZCRP-A and ZCRP-B are important in intracellular Zn trafficking and high-affinity transport, respectively. Metaproteomic detection of ZCRP-A and ZCRP-B homologs in a variety of eukaryotic protists along a South Pacific Ocean transect demonstrated that both proteins were more abundant in the euphotic zone (0-200m) where dZn and dCo were scarce. These findings describe widely distributed marine algal proteins linked to Zn and Co metabolism that together contribute to our understanding of Zn homeostasis in important marine phytoplankton.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_10:09:52.678.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026953
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Riss Kellogg
SpeciesList	scientific name: Thalassiosira pseudonana CCMP1335; NCBI TaxID: 296543; scientific name: Pseudo-nitzschia delicatissima; NCBI TaxID: 44447; scientific name: Chaetoceros sp.; NCBI TaxID: 49240; scientific name: Phaeodactylum tricornutum CCAP 1055/1; NCBI TaxID: 556484;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-27 16:01:30	ID requested
⏵ 1	2022-03-02 10:09:53	announced

Dataset with its publication pending

submitter keyword: diatoms, Thalassiosira pseudonana, Chaetoceros, Pseudo nitzschia, zinc, cobalt, proteomics

Mak A. Saito
contact affiliation	Woods Hole Oceanographic Institution, Marine Chemistry and Geochemistry Department
contact email	msaito@whoi.edu
lab head
Riss Kellogg
contact affiliation	Woods Hole Oceanographic Institution/ Massachusetts Institute of Technology (WHOI-MIT)
contact email	mkell0gg@mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026953
     1. Label: PRIDE project
     2. Name: Two novel zinc and cobalt-responsive proteins in marine eukaryotic phytoplankton and implications for Zn scarcity in the surface oceans