PXD026950

PXD026950 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative serum proteomic analysis in ischemic stroke patients
Description	Hemorrhagic transformation (HT), which occurs with or without reperfusion treatments (thrombolysis and/or thrombectomy), deteriorates the outcomes of ischemic stroke patients. It is essential to find clinically reliable biomarkers that can predict HT. In this study, we screened for potential serum biomarkers from an existing blood bank and database with 243 suspected acute ischemic stroke (AIS) patients. A total of 37 patients were enrolled, who were diagnosed as AIS but did not receive reperfusion treatment. They were divided into two groups based on whether they accompanied with HT or not (5 HT and 32 non-HT). Serum samples were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and compared under NCBInr database. A total of 647 proteins in sera samples were captured and the levels of 17 proteins (12 up-regulated and 5 down-regulated) were significantly different. These differentially expressed proteins were further categorized with Gene Ontology functional classification annotation and Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis into biological processes. Further protein-protein interaction analysis using String database discovered that, among the differential expressed proteins, 10 pairs of proteins were found to have crosstalk connections, which may have direct (physical) and indirect (functional) interactions for the development of HT. Our findings suggest that these differentially expressed proteins could serve as potential biomarkers for predicting HT after ischemic stroke.
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_00:34:24.161.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhifeng Qi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-27 15:51:31	ID requested
⏵ 1	2021-10-12 00:34:24	announced

Dataset with its publication pending

submitter keyword: iTRAQ, serum proteomics, hemorrhagic transformation, acute ischemic stroke

Zhifeng Qi
contact affiliation	Cerebrovascular Diseases Research Institute and Department of Neurology, Xuanwu Hospital of Capital Medical University
contact email	qizhifeng@xwh.ccmu.edu.cn
lab head
Zhifeng Qi
contact affiliation	Xuanwu Hospital of Capital Medical University
contact email	email_qzf@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026950
     1. Label: PRIDE project
     2. Name: iTRAQ-based quantitative serum proteomic analysis in ischemic stroke patients