PXD026939

PXD026939 is an original dataset announced via ProteomeXchange.

Title	Highly multiplexed quantitative phosphosite assay for biology and preclinical studies
Description	Reliable methods to quantify dynamic signaling changes across diverse pathways are needed to better understand the effects of disease and drug treatment in cells and tissues but are presently lacking. Here we present SigPath, a targeted mass spectrometry (MS) assay that measures 284 phosphosites in 200 phosphoproteins of biological interest. SigPath probes a broad swath of signaling biology with high throughput and quantitative precision. We applied the assay to investigate changes in phospho-signaling in drug-treated cancer cell lines, breast cancer preclinical models and human medulloblastoma tumors. In addition to validating previous findings, SigPath detected and quantified a large number of differentially regulated phosphosites newly associated with disease models and human tumors at baseline or with drug perturbation. Our results highlight the potential of SigPath to monitor phosphoproteomic signaling events and to nominate mechanistic hypotheses regarding oncogenesis, response and resistance to therapy.
HostingRepository	PanoramaPublic
AnnounceDate	2021-10-08
AnnouncementXML	Submission_2021-10-08_17:02:23.958.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Hasmik Keshishian
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Phospho; Carbamidomethyl; Oxidation; Label:13C(6)15N(1); Label:13C(5)15N(1); Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Quantiva

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-27 14:05:46	ID requested
⏵ 1	2021-10-08 17:02:24	announced

Keshishian H, McDonald ER, Mundt F, Melanson R, Krug K, Porter DA, Wallace L, Forestier D, Rabasha B, Marlow SE, Jane-Valbuena J, Todres E, Specht H, Robinson ML, Jean Beltran PM, Babur O, Olive ME, Golji J, Kuhn E, Burgess M, MacMullan MA, Rejtar T, Wang K, Mani DR, Satpathy S, Gillette MA, Sellers WR, Carr SA, A highly multiplexed quantitative phosphosite assay for biology and preclinical studies. Mol Syst Biol, 17(9):e10156(2021) [pubmed]

submitter keyword: Proteomics, post-translational modifications, targeted mass spectrometry, multiple reaction monitoring, selected reaction monitoring, breast cancer, medulloblastoma, CPTAC

Carr
contact affiliation	Broad Institute
contact email	scarr@broadinstitute.org
lab head
Hasmik Keshishian
contact affiliation	Broad Institute of MIT and Harvard
contact email	hasmik@broadinstitute.org
dataset submitter

Panorama Public dataset URI