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PXD026928

PXD026928 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMycoplasma gallisepticum WhiA knockdown and overexpression
DescriptionProteomes of Mycoplasma gallisepticum strains with overexpression and knockdown of WhiA transcription factor. The overexpression was introduced on a transposon vector carrying M. gallisepticum whiA gene with strong constitutive promoter and strong SD sequence. The knockdown was made by CRISPRi. dCas9 protein and sgRNA against whiA gene were introduced on a transposon vector.
HostingRepositoryPRIDE
AnnounceDate2021-06-25
AnnouncementXMLSubmission_2021-06-25_00:25:45.197.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAleksandr Zubov
SpeciesList scientific name: Mycoplasma gallisepticum S6; NCBI TaxID: 1006581;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-24 23:56:52ID requested
12021-06-25 00:25:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mycoplasma, transcription factor, WhiA, overexpression, knockdown
Contact List
Gleb Fisunov
contact affiliationLab of Proteome Research, Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
contact emailherr.romanoff@gmail.com
lab head
Aleksandr Zubov
contact affiliationLab of Proteome Research, Department of Molecular Biology and Genetics, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow
contact emailalexanderzubov@icloud.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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