PXD026927

PXD026927 is an original dataset announced via ProteomeXchange.

Title	Oestrogen Activates the MAP3K1 Cascade and -catenin to Promote Granulosa-Like Cell Fate in a Human Testis-Derived Cell Line
Description	: Sex determination triggers the differentiation of the bi-potential gonad into either an ovary or testis. In non-mammalian vertebrates, the presence or absence of oestrogen dictates gonad differ-entiation, while in mammals, this mechanism has been supplanted by the testis determining SRY gene. Exogenous oestrogen can override this genetic trigger to shift somatic cell fate in the gonad towards ovarian developmental pathways by limiting the bioavailability of the key testis factor SOX9 within somatic cells. Our previous work has implicated the MAPK pathway in mediating the rapid cellular response to oestrogen. We performed proteomic and phosphoproteomic anal-yses to investigate the precise mechanism through which oestrogen impacts these pathways to ac-tivate -catenin—a factor essential for ovarian development. We show that oestrogen can activate -catenin within 30 minutes, concomitant with the cytoplasmic retention of SOX9. This occurs through changes to the MAP3K1 cascade, suggesting this pathway is a mechanism through which oestrogen influences gonad somatic cell fate. We demonstrate that oestrogen can promote the shift from SOX9 pro-testis activity to -catenin pro-ovary activity through activation of MAP3K1. Our findings define a previously unknown mechanism through which oestrogen can promote a switch in gonad somatic cell fate and provided novel insights into the impacts of exogenous oestrogen exposure on the testis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:56:08.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Stewart
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-24 23:55:37	ID requested
⏵ 1	2022-02-17 04:56:09	announced

Stewart MK, Bernard P, Ang CS, Mattiske DM, Pask AJ, -Catenin to Promote Granulosa-like Cell Fate in a Human Testis-Derived Cell Line. Int J Mol Sci, 22(18):(2021) [pubmed]

submitter keyword: oestrogen, SOX9, beta-catenin, MAP3K1

Andrew John Pask
contact affiliation	School of BioSciences, The University of Melbourne, Victoria, Australia
contact email	ajpask@unimelb.edu.au
lab head
Melanie Stewart
contact affiliation	Peter Maccallum Cancer Centre
contact email	melanie.stewart@petermac.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026927

PRIDE project URI

• PRIDE
  1. PXD026927
     1. Label: PRIDE project
     2. Name: Oestrogen Activates the MAP3K1 Cascade and -catenin to Promote Granulosa-Like Cell Fate in a Human Testis-Derived Cell Line