PXD026926

PXD026926 is an original dataset announced via ProteomeXchange.

Title	Interactome of a TurboID-tagged BRD9 in the presence or absence of IFN-a2
Description	BRD9 was identified in a genome-wide screen for genes regulating the response to interferon (IFN) in a A549 based reporter cell line. Subsequent experiments determined an involvement of BRD9 in the transcriptional regulation of Interferon-stimulated genes (ISGs) expression following stimulation with IFN-a2. The aim of this proximity-labelling experiments was to gain a more mechanistic understanding of BRD9 recruitment during the IFN signal transduction using A549 cells stably transduced with BRD9-TurboID and mCherry-TurboID fusion proteins. The BRD9 interactome in the absence of IFN- a2 was determined. We found that following IFN-a2 treatment, STAT2 significantly associates with BRD9-TurboID.
HostingRepository	PRIDE
AnnounceDate	2021-08-17
AnnouncementXML	Submission_2021-08-17_03:03:26.676.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacob Börold
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-24 23:54:48	ID requested
⏵ 1	2021-08-17 03:03:27	announced

Dataset with its publication pending

submitter keyword: TurboID, BRD9, A549, STAT2, BRD9-TurboID, proximity labeling, IFN-signalling

Benjamin Geoffrey Hale
contact affiliation	University of Zurich, Institute for Medical Virology, Switzerland
contact email	hale.ben@virology.uzh.ch
lab head
Jacob Börold
contact affiliation	University of Zurich
contact email	boerold.jacob@virology.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026926

PRIDE project URI

• PRIDE
  1. PXD026926
     1. Label: PRIDE project
     2. Name: Interactome of a TurboID-tagged BRD9 in the presence or absence of IFN-a2