PXD026923

PXD026923 is an original dataset announced via ProteomeXchange.

Title	Cell-matrix adhesion contributes to permeability control in human colon organoids
Description	Background and aims: Cell-cell adhesion structures (desmosomes and, especially, tight junctions) are known to play important roles in control of transepithelial permeability in the colon. The involvement of cell-matrix interactions in permeability control is less clear. The goals of the present study were to: i) determine if disruption of colon epithelial cell interactions with the extracellular matrix alters permeability control and ii) determine if increasing the elaboration of protein components of cell-matrix adhesion complexes improves permeability control and mitigates the effects of cell-matrix disruption. Results: Treatment of colon organoids with Aquamin® increased the expression of multiple basement membrane and hemidesmosomal proteins as well as keratin 8 and 18. TEER values were higher in the presence of Aquamin® than they were under control conditions. Conclusions: These findings provide evidence that cell-matrix interactions contribute to permeability control in the colon. They suggest that the elaboration of proteins important to cell-matrix interactions can be increased in human colon organoids by exposure to a multi-mineral natural product. Increasing the elaboration of such proteins may help to mitigate the consequences of disrupting cell-matrix interactions on permeability control.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-04-14_07:01:32.256.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Aslam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-24 23:50:06	ID requested
1	2022-03-14 04:58:26	announced
⏵ 2	2022-04-14 07:01:33	announced	2022-04-14: Updated publication reference for PubMed record(s): 35360746.

Varani J, McClintock SD, Aslam MN, Cell-Matrix Interactions Contribute to Barrier Function in Human Colon Organoids. Front Med (Lausanne), 9():838975(2022) [pubmed]

submitter keyword: Colon, gut barrier, basement membrane, colon organoids, intestinal permeabilty

Muhammad N Aslam
contact affiliation	Departmemnt of Pathology, University of Michigan
contact email	mnaslam@med.umich.edu
lab head
Muhammad Aslam
contact affiliation	University of Michigan
contact email	mnaslam@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026923
     1. Label: PRIDE project
     2. Name: Cell-matrix adhesion contributes to permeability control in human colon organoids