PXD026906

PXD026906 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of serum samples of paracoccidioidomycosis patients with severe pulmonary sequel
Description	Pulmonary sequelae (PS) in patients with chronic paracoccidioidomycosis (PCM) typically include pulmonary fibrosis and emphysema. Knowledge of the molecular pathways involved in PS of PCM is required for treatment and biomarker identification. This non-concurrent cohort study included 29 patients with pulmonary PCM that were followed before and after treatment. From this group, 17 patients evolved to mild/ moderate PS and 12 evolved severe PS. Sera from patients were evaluated before treatment and at clinical cure, serological cure, and apparent cure. A nanoACQUITY UPLC-Xevo QT MS system and PLGS software were used to identify serum differentially expressed proteins, which were then categorized using Cytoscape software and the Reactome pathway database. Seventy-two differentially expressed serum proteins were identified in patients with severe PS compared with patients with mild/moderate PS. Most proteins altered in severe PS were involved in wound healing, inflammatory response, and oxygen transport pathways. Before treatment and at clinical cure, signaling proteins participating in wound healing, complement cascade, cholesterol transport and retinoid metabolism pathways were downregulated in patients with severe PS, whereas signaling proteins in gluconeogenesis and gas exchange pathways were upregulated. At serological cure, the pattern of protein expression reversed. At apparent cure pathways related with tissue repair (fibrosis) became downregulated, and pathway related oxygen transport became upregulated. Additionally, we identified 11 proteins as candidate biomarkers for severe PS. Development of severe PS is related to increased expression of proteins involved in anabolism (gluconeogenesis and oxygen exchange), indicative of the greater cellular activity and replication associated with early dysregulation of wound healing and aberrant tissue repair. Our findings provide new targets to study mechanisms of PS in PCM, as well as potential biomarkers.
HostingRepository	PRIDE
AnnounceDate	2021-08-26
AnnouncementXML	Submission_2021-08-26_04:26:19.971.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amanda Ribeiro dos Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-24 03:41:05	ID requested
⏵ 1	2021-08-26 04:26:20	announced

Santos ARD, Dionizio A, Fernandes MDS, Buzalaf MAR, Pereira B, Donanzam DFA, Ribeiro SM, Paniago AMM, Cavalcante RS, Mendes RP, Venturini J, Proteomic analysis of serum samples of paracoccidioidomycosis patients with severe pulmonary sequel. PLoS Negl Trop Dis, 15(8):e0009714(2021) [pubmed]

submitter keyword: Paracoccidioides brasiliensis, pulmonary fibrosis, molecular analysis

Amanda Ribeiro dos Santos
contact affiliation	Universidade Federal do Mato Grosso do Sul- UFMS
contact email	amanda_ribeiro@ufms.br
lab head
Amanda Ribeiro dos Santos
contact affiliation	Universidade Federal do Mato Grosso do Sul
contact email	amanda_ribeiro@ufms.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026906
     1. Label: PRIDE project
     2. Name: Proteomic analysis of serum samples of paracoccidioidomycosis patients with severe pulmonary sequel