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PXD026905

PXD026905 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of T-cruzi experimentally infected hamster cheeck pouch
DescriptionMast cells (MCs) were implicated in tissue remodelling in hamster models of heart inflammatory diseases. Here we asked whether MCs promote inflammatory neovascularization in hamster cheek pouch (HCP) parasitized by Trypanosoma cruzi, the protozoan that causes Chagas disease. Intravital microscopy (IVM) performed 3 days after inoculation of Dm28c trypomastigote (GFP-TCTs) showed a subtle infiltration of rhodamine-labeled leukocytes. Interestingly, the baseline levels of FITC-dextran leakage (3 dpi) were consistently increased, raising the possibility that intracellular amastigotes may coopt the low-grade inflammation to facilitate the delivery of plasma-borne nutrients during the period in which host cells must metabolically sustain parasite division. Although proangiogenic indexes were only mildly stimulated at 3 dpi, we found a positive correlation with transcription of proinflammatory cytokines (pro-IL- and IFN-). Having established 3 dpi as a timepoint in which vascular remodeling was already measurable, we next compared the proteomic profiles of parasitized HCP with those of internal controls (PBS injected 3 days earlier in the contralateral pouch) we found 87 proteins upregulated, and 17 were downregulated in the parasitized HCP. Minor changes in protein expression were observed in response to PBS injection (2 upregulated and 12 downregulated proteins. Congruent with the increased density of MCs observed in parasitized HCP (histological analysis), the MC protease 1 (chymase) stood out as the most abundant sequence. Interestingly, toluidine blue staining appointed clusters of MCs degranulating at 3 dpi. Noteworthy, two plasma proteins (complement C3 and serum albumin) were also identified as major proteins, further suggesting that leaky capillaries irrigated the HCP at early stages (3 dpi) of infection . Detection of cytokines or classical proangiogenic factors was beyond the sensitivity of our methods.
HostingRepositoryPRIDE
AnnounceDate2022-04-04
AnnouncementXMLSubmission_2022-04-03_19:24:48.798.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterProteomics Unit
SpeciesList scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-24 03:40:56ID requested
12022-04-03 19:24:49announced
Publication List
Vellasco L, Svensj, ö E, Bulant CA, Blanco PJ, Nogueira F, Domont G, de Almeida NP, Nascimento CR, Silva-Dos-Santos D, Carvalho-Pinto CE, Medei EH, Almeida IC, Scharfstein J, Orchestrates Inflammatory Neovascularization via Activation of the Mast Cell Chymase Pathway. Pathogens, 11(2):(2022) [pubmed]
Keyword List
submitter keyword: hamster, cheeck pouch, proteomics
Contact List
Fabio Nogueira
contact affiliationFederal University of Rio de Janeiro
contact emailfabiocsn@gmail.com
lab head
Proteomics Unit
contact affiliationUFRJ
contact emailunidadeproteomica@gmail.com
dataset submitter
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