PXD026886 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Median Based Absolute Quantification using Fully Unlabelled Generic Internal Standard |
Description | By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide standards or on a semi-empirical calibration against the average abundance of peptides chosen from arbitrary selected standard proteins. Here we developed a generic protein standard FUGIS (Fully unlabelled Generic Internal Standard) that requires no isotopic labelling, synthesis of standards or external calibration and is applicable to proteins of any organismal origin. FUGIS is co-digested with analysed proteins and enables their absolute quantification in the same LC-MS/MS run. By using FUGIS, median based absolute quantification (MBAQ) workflow provides similar quantification accuracy compared to isotopically-labelled peptide standards and outperforms methods based on external calibration or selection of best ionized reporter peptides (Top3 quantification) with a median quantification error less than 15% |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-01 |
AnnouncementXML | Submission_2021-12-01_08:15:48.954.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bharath Kumar Raghuraman |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-23 09:17:03 | ID requested | |
⏵ 1 | 2021-12-01 08:15:49 | announced | |
Publication List
Raghuraman BK, Bogdanova A, Moon H, Rzagalinski I, Geertsma ER, Hersemann L, Shevchenko A, Median-Based Absolute Quantification of Proteins Using Fully Unlabeled Generic Internal Standard (FUGIS). J Proteome Res, 21(1):132-141(2022) [pubmed] |
Keyword List
submitter keyword: Absolute quantification |
Contact List
Andrej Shevchenko |
contact affiliation | Max Planck Institute of Molecular Cell Biology and Genetic |
contact email | shevchenko@mpi-cb.de |
lab head | |
Bharath Kumar Raghuraman |
contact affiliation | Max Planck Institute of Molecular Cell Biology and Genetics |
contact email | raghuram@mpi-cbg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026886
- Label: PRIDE project
- Name: Median Based Absolute Quantification using Fully Unlabelled Generic Internal Standard