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PXD026886

PXD026886 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMedian Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
DescriptionBy reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide standards or on a semi-empirical calibration against the average abundance of peptides chosen from arbitrary selected standard proteins. Here we developed a generic protein standard FUGIS (Fully unlabelled Generic Internal Standard) that requires no isotopic labelling, synthesis of standards or external calibration and is applicable to proteins of any organismal origin. FUGIS is co-digested with analysed proteins and enables their absolute quantification in the same LC-MS/MS run. By using FUGIS, median based absolute quantification (MBAQ) workflow provides similar quantification accuracy compared to isotopically-labelled peptide standards and outperforms methods based on external calibration or selection of best ionized reporter peptides (Top3 quantification) with a median quantification error less than 15%
HostingRepositoryPRIDE
AnnounceDate2021-12-01
AnnouncementXMLSubmission_2021-12-01_08:15:48.954.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBharath Kumar Raghuraman
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-23 09:17:03ID requested
12021-12-01 08:15:49announced
Publication List
Raghuraman BK, Bogdanova A, Moon H, Rzagalinski I, Geertsma ER, Hersemann L, Shevchenko A, Median-Based Absolute Quantification of Proteins Using Fully Unlabeled Generic Internal Standard (FUGIS). J Proteome Res, 21(1):132-141(2022) [pubmed]
Keyword List
submitter keyword: Absolute quantification
Contact List
Andrej Shevchenko
contact affiliationMax Planck Institute of Molecular Cell Biology and Genetic
contact emailshevchenko@mpi-cb.de
lab head
Bharath Kumar Raghuraman
contact affiliationMax Planck Institute of Molecular Cell Biology and Genetics
contact emailraghuram@mpi-cbg.de
dataset submitter
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Dataset FTP location
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