PXD026886

PXD026886 is an original dataset announced via ProteomeXchange.

Title	Median Based Absolute Quantification using Fully Unlabelled Generic Internal Standard
Description	By reporting molar abundances of proteins, absolute quantification determines their stoichiometry in complexes, pathways or networks and also relates them to abundances of non-protein biomolecules. Typically, absolute quantification relies either on protein- specific isotopically labelled peptide standards or on a semi-empirical calibration against the average abundance of peptides chosen from arbitrary selected standard proteins. Here we developed a generic protein standard FUGIS (Fully unlabelled Generic Internal Standard) that requires no isotopic labelling, synthesis of standards or external calibration and is applicable to proteins of any organismal origin. FUGIS is co-digested with analysed proteins and enables their absolute quantification in the same LC-MS/MS run. By using FUGIS, median based absolute quantification (MBAQ) workflow provides similar quantification accuracy compared to isotopically-labelled peptide standards and outperforms methods based on external calibration or selection of best ionized reporter peptides (Top3 quantification) with a median quantification error less than 15%
HostingRepository	PRIDE
AnnounceDate	2021-12-01
AnnouncementXML	Submission_2021-12-01_08:15:48.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bharath Kumar Raghuraman
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-23 09:17:03	ID requested
⏵ 1	2021-12-01 08:15:49	announced

Raghuraman BK, Bogdanova A, Moon H, Rzagalinski I, Geertsma ER, Hersemann L, Shevchenko A, Median-Based Absolute Quantification of Proteins Using Fully Unlabeled Generic Internal Standard (FUGIS). J Proteome Res, 21(1):132-141(2022) [pubmed]

submitter keyword: Absolute quantification

Andrej Shevchenko
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetic
contact email	shevchenko@mpi-cb.de
lab head
Bharath Kumar Raghuraman
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics
contact email	raghuram@mpi-cbg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026886
     1. Label: PRIDE project
     2. Name: Median Based Absolute Quantification using Fully Unlabelled Generic Internal Standard