PXD026880

PXD026880 is an original dataset announced via ProteomeXchange.

Title	Nascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesisNascent alt-protein chemoproteomics reveals a repressor of pre-60S assembly
Description	Many unannotated microproteins and alternative proteins (alt-proteins) have recently been found to be co-encoded with canonical proteins, but few of their functions are known. Motivated by the hypothesis that alt-proteins undergoing regulated synthesis could play important cellular roles, we developed a chemoproteomic pipeline to identify nascent alt-proteins in human cells. We identified 22 actively translated alt-proteins or N-terminal extensions, one of which is post-transcriptionally upregulated by DNA damage stress. We further defined cell cycle-regulated MINAS-60 (MIcroprotein that Negatively regulates ASsembly of the pre-60S ribosomal subunit), a nucleolar alt-protein co-encoded with human RBM10. Depletion of MINAS-60 increases the amount of cytoplasmic 60S ribosomal subunit, upregulating global protein synthesis and cell proliferation. Mechanistically, MINAS-60 represses the rate of late-stage pre-60S assembly and export to the cytoplasm. Together, these results implicate MINAS-60 as a repressor of pre-60S maturation, and demonstrate that chemoproteomics can enable functional hypothesis generation for uncharacterized alt-proteins.
HostingRepository	PRIDE
AnnounceDate	2022-09-13
AnnouncementXML	Submission_2022-09-13_12:53:42.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiongwen cao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-23 08:00:31	ID requested
⏵ 1	2022-09-13 12:53:44	announced

Cao X, Khitun A, Harold CM, Bryant CJ, Zheng SJ, Baserga SJ, Slavoff SA, Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor. Nat Chem Biol, 18(6):643-651(2022) [pubmed]

submitter keyword: human HEK 293T, LC-MS/MS

Sarah A. Slavoff
contact affiliation	Yale University
contact email	sarah.slavoff@yale.edu
lab head
xiongwen cao
contact affiliation	Yale University, Chemical Biology Institute
contact email	xiongwen.cao@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD026880

PRIDE project URI

• PRIDE
  1. PXD026880
     1. Label: PRIDE project
     2. Name: Nascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesisNascent alt-protein chemoproteomics reveals a repressor of pre-60S assembly