To support quantitative data analysis, we have developed a software, peakfit, that fits acquired chromatographic data to the log-normal peak equation and reports the calculated peak parameters. To demonstrate the capabilities of this approach, we provide hereby four example datasets: (1) 15 QCs of a PRM assay targeting the three most common isoforms of Apolipoprotein E (E2, E3, and E4); (2) PRM data of samples with 6 different ApoE phenotypes (E2/2, E2/3, E2/4, E3/3, E3/4, and E4/4); (3) shotgun run of a commercial HeLa digest to demonstrate processing of MS1 data, and (4) a quality control peptide mix to demonstrate assessment of chromatographic performance on basis of the base peak chromatogram.