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PXD026872

PXD026872 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA YAP/TAZ-TEAD signaling module links endothelial nutrient acquisition to angiogenic growth
DescriptionAngiogenesis, the process by which endothelial cells (ECs) form new blood vessels from existing ones, is intimately linked to the tissue's metabolic milieu and often occurs at nutrient-deficient sites. However, ECs rely on sufficient metabolic resources to support growth and proliferation. How endothelial nutrient acquisition and usage are regulated is unknown. Here we show that these processes are dictated by YAP/TAZ-TEAD – a transcriptional module whose function is highly responsive to changes in the tissue environment. ECs lacking YAP/TAZ or their transcriptional partners, TEAD1, 2, and 4 fail to divide, resulting in stunted vascular growth in mice. Conversely, activation of TAZ, the more abundant paralogue in ECs, boosts proliferation, leading to vascular hyperplasia. We find that YAP/TAZ promote angiogenesis by fueling nutrient mTORC1 signaling. By orchestrating the transcription of a repertoire of cell-surface transporters, YAP/TAZ-TEAD stimulate the import of amino acids and other essential nutrients, thereby enabling mTORC1 pathway activation. Dissociating mTORC1 from these nutrient inputs – elicited by the loss of Rag GTPases – inhibits mTORC1 activity and prevents YAP/TAZ-dependent vascular growth. These findings define a pivotal role for YAP/TAZ-TEAD in steering endothelial mTORC1 and illustrate the essentiality of coordinated nutrient fluxes in the vasculature.
HostingRepositoryPRIDE
AnnounceDate2022-05-08
AnnouncementXMLSubmission_2022-05-08_06:00:58.359.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterScientific Service Group Biomolecular Mass Spectrometry
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-22 22:59:17ID requested
12022-05-08 06:00:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: YAP/TAZ-TEAD signaling, endothelial cells, angiogenesis, nutrient acquisition, mTORC1
Contact List
Michael Potente
contact affiliationAngiogenesis & Metabolism Laboratory, Max Planck Institut for Heart and Lung Research, 61231 Bad Nauheim, Germany
contact emailmichael.potente@mpi-bn.mpg.de
lab head
Scientific Service Group Biomolecular Mass Spectrometry
contact affiliationMax Planck Institute for Heart and Lung Research
contact emailproteomics@mpi-bn.mpg.de
dataset submitter
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Dataset FTP location
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