PXD026866

PXD026866 is an original dataset announced via ProteomeXchange.

Title	A molecular network of conserved factors keeps ribosomes dormant in the egg
Description	Ribosomes are produced in large quantities during oogenesis and stored in the egg. However, the egg and early embryo are translationally inactive. How translation is activated during embryogenesis is poorly understood. Using mass-spectrometry and cryo-EM analyses of ribosomes isolated from zebrafish and Xenopus eggs and embryos, we find that ribosomes transition from a dormant to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four factors that form two modules: a Habp4-eEF2 module that stabilizes ribosome levels and a Dap1b/Dapl1-eIF-5a module that represses translation. Dap/Dap1b is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Thus, a developmentally programmed, unique ribosome state plays a key role in ribosome storage and translational repression in the egg.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:00:34.181.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuel Matzinger
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-22 08:08:23	ID requested
1	2022-11-03 15:44:57	announced
2	2023-02-02 04:17:23	announced	2023-02-02: Updated project metadata.
⏵ 3	2023-11-14 08:00:35	announced	2023-11-14: Updated project metadata.

Leesch F, Lorenzo-Orts L, Pribitzer C, Grishkovskaya I, Roehsner J, Chugunova A, Matzinger M, Roitinger E, Bela, č, i, ć K, Kandolf S, Lin TY, Mechtler K, Meinhart A, Haselbach D, Pauli A, A molecular network of conserved factors keeps ribosomes dormant in the egg. Nature, 613(7945):712-720(2023) [pubmed]

submitter keyword: cryo-EM, cross-linking, Xenopus, mass-spectrometry, ribosome,Zebrafish

Andrea Pauli
contact affiliation	Institure of Molecular Pathology
contact email	andrea.pauli@imp.ac.at
lab head
Manuel Matzinger
contact affiliation	IMP / University of Vienna
contact email	manuel.matzinger@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD026866

PRIDE project URI

• PRIDE
  1. PXD026866
     1. Label: PRIDE project
     2. Name: A molecular network of conserved factors keeps ribosomes dormant in the egg