PXD026833

PXD026833 is an original dataset announced via ProteomeXchange.

Title	A role for PchHI as an ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa
Description	Iron is an essential nutrient for bacterial growth but poorly bioavailable. To scavenge ferric iron present in their environment, bacteria synthesize and secrete siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. Once having captured a ferric iron, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and afterwards by the inner membrane permease FptX. Here using molecular biology, 55Fe uptake assays and LC-MS/MS quantification of PCH in the different bacterial cell fractions, we show that (i) PCH (probably under its PCH-Fe form) is able to rich bacterial periplasm and cytoplasm when both FptA and FptX are expressed, and (ii) that PchHI (a heterodimeric ABC transporter) plays a role in the translocation of siderophore-free iron siderophore-free iron across the inner membrane into the cytoplasm. Consequently, probably the first fraction of PCH-Fe internalized by FptA may be transported further by FptX in the bacterial cytoplasm to activate the transcriptional regulator PchR, regulating the transcription of all genes of the PCH pathway. The further fractions of PCH-Fe transported by FptA may dissociate in the bacterial periplasm by an unknown mechanism, with the siderophore-free iron being transported into the cytoplasm by PchHI.
HostingRepository	PRIDE
AnnounceDate	2022-03-15
AnnouncementXML	Submission_2022-03-15_01:40:50.722.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026833
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-21 03:04:10	ID requested
⏵ 1	2022-03-15 01:40:51	announced

Roche B, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Br, ö, nstrup M, Mislin GLA, Schalk IJ, A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. Environ Microbiol, 24(2):866-877(2022) [pubmed]

submitter keyword: iron, siderophore, pyochelin, Pseudomonas, PchHI

Isabelle Schalk
contact affiliation	CNRS, UMR7242, ESBS, Bld Sébastien Brant, F-67412 Illkirch, France. Université de Strasbourg, UMR7242, ESBS, Bld Sébastien Brant, F-67412 Illkirch, France.
contact email	schalk@unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026833
     1. Label: PRIDE project
     2. Name: A role for PchHI as an ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa