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PXD026833

PXD026833 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA role for PchHI as an ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa
DescriptionIron is an essential nutrient for bacterial growth but poorly bioavailable. To scavenge ferric iron present in their environment, bacteria synthesize and secrete siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. Once having captured a ferric iron, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and afterwards by the inner membrane permease FptX. Here using molecular biology, 55Fe uptake assays and LC-MS/MS quantification of PCH in the different bacterial cell fractions, we show that (i) PCH (probably under its PCH-Fe form) is able to rich bacterial periplasm and cytoplasm when both FptA and FptX are expressed, and (ii) that PchHI (a heterodimeric ABC transporter) plays a role in the translocation of siderophore-free iron siderophore-free iron across the inner membrane into the cytoplasm. Consequently, probably the first fraction of PCH-Fe internalized by FptA may be transported further by FptX in the bacterial cytoplasm to activate the transcriptional regulator PchR, regulating the transcription of all genes of the PCH pathway. The further fractions of PCH-Fe transported by FptA may dissociate in the bacterial periplasm by an unknown mechanism, with the siderophore-free iron being transported into the cytoplasm by PchHI.
HostingRepositoryPRIDE
AnnounceDate2022-03-15
AnnouncementXMLSubmission_2022-03-15_01:40:50.722.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD026833
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-21 03:04:10ID requested
12022-03-15 01:40:51announced
Publication List
Roche B, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Br, ö, nstrup M, Mislin GLA, Schalk IJ, A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. Environ Microbiol, 24(2):866-877(2022) [pubmed]
Keyword List
submitter keyword: iron, siderophore, pyochelin, Pseudomonas, PchHI
Contact List
Isabelle Schalk
contact affiliationCNRS, UMR7242, ESBS, Bld Sébastien Brant, F-67412 Illkirch, France. Université de Strasbourg, UMR7242, ESBS, Bld Sébastien Brant, F-67412 Illkirch, France.
contact emailschalk@unistra.fr
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
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