PXD026830

PXD026830 is an original dataset announced via ProteomeXchange.

Title	Co-immunoprecipitation of G-quadruplex-containing Core Promoter and nanoLC-FAMIS-MS/MS
Description	G-quadruplexes (G4s) are noncanonical DNA secondary structures formed through the self-association of guanines, and they are distributed widely across the genome. G4 participates in multiple biological processes including gene transcription, and G4-targeted ligands serve as potential therapeutic agents for DNA-targeted therapies. However, genome-wide studies of the exact roles of G4s in transcriptional regulation are still lacking. We found that drug-induced promoter-proximal RNA polymerase II pausing promotes nearby G4 formation, and oppositely, G4 stabilization by G4-targeted ligands globally reduces RNA polymerase II occupancy at gene promoters as well as nascent RNA synthesis. To study the underlying mechanisms by which native G4 affects transcriptional regulation, we annealed the biotin-labeled core promoter DNA to form G4s and performed pull-down assays with nuclear extraction proteins in the presence or absence of TMPyP4. Mass spectrometry analysis was performed to identify the interacting proteins with G4-forming core promoter DNA.
HostingRepository	PRIDE
AnnounceDate	2021-07-19
AnnouncementXML	Submission_2021-07-19_03:36:09.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Conghui Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-21 03:03:57	ID requested
⏵ 1	2021-07-19 03:36:10	announced

Dataset with its publication pending

submitter keyword: Human, G-quadruplex, core promoter, nuclear protein, co-immunoprecipitation, nanoLC-FAMIS-MS/MS

Kaiwei Liang
contact affiliation	Department of pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
contact email	KWliang@whu.edu.cn
lab head
Conghui Li
contact affiliation	Wuhan University
contact email	liconghui@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD026830

PRIDE project URI

• PRIDE
  1. PXD026830
     1. Label: PRIDE project
     2. Name: Co-immunoprecipitation of G-quadruplex-containing Core Promoter and nanoLC-FAMIS-MS/MS